Estrogen downregulates the number of caveolae and the level of caveolin in uterine smooth muscle
Estrogen downregulates the number of caveolae and the level of caveolin in uterine smooth muscle
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DOI：
10.1006/cbir.2001.0769
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发表时间：
2001-01-01
影响因子：
3.9
通讯作者：
Müllner, N
中科院分区：
文献类型：
--
作者：
Turi, A;Kiss, AL;Müllner, N
雌激素和孕激素在调节子宫功能的同时，也调节小窝的数量和小窝蛋白的水平。大量的小窝，以及小窝蛋白-1和小窝蛋白-2亚型的表达升高的卵巢切除（OVX）大鼠子宫肌层中检测。17 β-雌二醇（E2）具有下调作用：用E2（5 μ g/动物）处理OVX大鼠使小窝的形成减少约1.5 μ g/只。百分之九十蛋白质印迹清楚地表明膜小窝蛋白-1和-2含量的减少。单独的孕酮治疗（2.5 mg/只动物）没有引起任何实质性变化，但阻止了雌激素的作用。对照实验表明，Na+/K+-ATP酶的数量，质膜蛋白质排除小窝，不下调E2。给予纯雌激素受体（ER α）拮抗剂ICI 182，780（1 mg/动物）不仅补偿了E2的抑制作用，而且进一步增加了OVX大鼠子宫肌层中小窝蛋白-1的水平，并促进了小窝的形成，接近70%。相反，部分拮抗剂他莫昔芬（1毫克/动物）模仿雌激素的作用。小窝蛋白的量在怀孕期间也发生了变化。在怀孕的前半期，小窝蛋白的表达受到抑制，但它逐渐增加，直到分娩。我们的研究结果表明，子宫小窝的形成和数量的影响，在激素依赖的方式由子宫的生理状态。(C)北京：科学出版社.
Estrogen and progesterone, while regulating uterine functions, also regulate the number of caveolae and the level of caveolin. Large numbers of caveolae, as well as elevated expression of caveolin-1 and caveolin-2 isoforms in the myometrium of ovariectomised (OVX) rats were detected. 17 beta -estradiot (E2) has a downregulating effect: the treatment of OVX rats with E2 (5 mug/animal) reduced the formation of caveolae by approx. 90%. Western blots clearly demonstrated the reduction of membrane caveolin-1 and -2 content. Progesterone treatment (2.5 mg/animal) alone did not cause any substantial change, but prevented the effect of estrogen. Control experiments showed that the quantity of Na+/K+-ATPase, a plasma membrane protein excluded from caveolae, was not downregulated by E2. The administration of the pure estrogen receptor (ER alpha) antagonist ICI 182,780 (1 mg/animal) not only compensated for the inhibitory effect of E2, but further increased the level of caveolin-1 in the myometrium of OVX rats and facilitated the formation of caveolae by similar to 70%. In contrast, the partial antagonist tamoxifen (I mg/animal) mimicked the effect of estrogen. The amount of caveolin also changed during pregnancy. During the first half of pregnancy the expression of caveolin was suppressed, but it gradually increased until delivery. Our results indicate that the formation and number of caveolae are influenced by the physiological state of the uterus in a hormone dependent manner. (C) 2001 Academic Press.