Localization of integrin receptors for fibronectin, collagen, and laminin in human skin. Variable expression in basal and squamous cell carcinomas.
Localization of integrin receptors for fibronectin, collagen, and laminin in human skin. Variable expression in basal and squamous cell carcinomas.
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人体皮肤中纤连蛋白、胶原蛋白和层粘连蛋白整合素受体的定位。
DOI：
10.1172/jci114379
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Uitto,J
中科院分区：
文献类型：
--
作者：
Peltonen,J;Larjava,H;Jaakkola,S;Gralnick,H;Akiyama,SK;Yamada,SS;Yamada,KM;Uitto,J
利用识别 β1、α2、α3 或 α5 亚基的抗体，通过间接免疫荧光检查人类皮肤中的 VLA 整合素。用β1、α2或α3亚基抗体对胎儿、新生儿或成人皮肤进行染色，得到基本上相似的染色模式：强烈染色与表皮、毛囊和血管壁的基底层相关。 α5亚基仅在胎儿皮肤的表皮和毛囊内根鞘中检测到。在表皮中，β1亚基的染色反应不仅出现在与基底膜区交界的部位，而且还出现在这些细胞的整个外围。我们推测这些受体可能在细胞间相互作用中具有以前未被识别的功能，或者这些发现可能表明角质形成细胞的细胞间隙中存在以前未被识别的配体。对九个结节性基底细胞癌的检查显示，肿瘤岛周围存在抗β1和抗α3抗体的显着染色反应。相比之下，五种鳞状细胞癌的染色显示整合素不存在或表达发生改变和变化。因此，基质成分及其受体可能参与调节人类皮肤的生长、发育和组织。
VLA integrins in human skin were examined by indirect immunofluorescence utilizing antibodies recognizing the beta 1, alpha 2, alpha 3, or alpha 5 subunits. Staining of fetal, newborn, or adult skin with antibodies to beta 1, alpha 2, or alpha 3 subunits gave essentially similar staining patterns: intense staining was associated with the basal layer of the epidermis, hair follicles, and blood vessel walls. The alpha 5 subunit could be detected only in epidermis and the inner root sheath of hair follicles in fetal skin. In epidermis, the staining reaction for the beta 1 subunit was not only found in sites interfacing with the basement membrane zone, but also around the entire periphery of these cells. We speculate that these receptors might have previously unrecognized functions in cell-cell interactions or that these findings may suggest the presence of previously unrecognized ligands in the intercellular spaces of keratinocytes. Examination of nine nodular basal cell carcinomas revealed a prominent staining reaction with anti-beta 1 and anti-alpha 3 antibodies at the periphery of the tumor islands. In contrast, staining of five squamous cell carcinomas revealed either the absence of integrins or altered and variable expression. Thus, matrix components and their receptors may participate in modulation of growth, development, and organization of human skin.Images