Whole metagenome sequencing of chlorinated drinking water distribution systems
Whole metagenome sequencing of chlorinated drinking water distribution systems
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DOI：
10.1039/c8ew00395e
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发表时间：
2018-12-01
影响因子：
5
通讯作者：
Boxall, Joby B.
中科院分区：
文献类型：
--
作者：
Douterelo, Isabel;Calero-Preciado, Carolina;Boxall, Joby B.
有一个爆炸性的研究存在于饮用水分配系统（DWDS）中的微生物。然而，以前的研究主要集中在分类组成和鲜为人知的实际基因组成的宏基因组的DWDS和他们的功能或这些信息是否可以用于遗传分析和监测过程中发生的DWDS。我们在这里首次使用全宏基因组鸟枪测序，从生物膜和散装水样品从操作，氯化DWDS的微生物群落。基因含量分析揭示了特定生境（生物膜与水）的生物体以及基因功能方面的差异，这表明适应特定的环境。此外，在生物膜内优先确定了几种抗性机制，包括与预防和修复消毒剂自由基诱导的损伤和抗生素抗性相关的基因。这项研究强调了这些信息的潜力，以帮助保护饮用水的质量和安全，在未来的进一步研究和更广泛的应用。
There has been an explosion of research into the microorganisms present within drinking water distribution systems (DWDS). However, previous studies have focused mainly on the taxonomic composition and little is known about the actual genes composing the metagenomes of DWDS and their function or whether such information could be used for genetic profiling and monitoring processes taking place in DWDS. We use here for the first time whole metagenome shotgun sequencing to characterise microbial communities from both biofilm and bulk water samples from operational, chlorinated DWDS. Gene content analysis revealed habitat-specific (biofilm vs. water) differences in terms of organisms as well as gene functions, suggesting adaptation to specific environments. In addition, several resistance mechanisms were identified preferentially within biofilms, including genes associated with the prevention and repair of disinfectant radical-induced damage and antibiotic resistance. This research highlights the potential of such information to help protect drinking water quality and safety in the future following further research and wider application.