Genome-wide SNP analysis identifies major QTL for Salmonella colonization in the chicken
Genome-wide SNP analysis identifies major QTL for Salmonella colonization in the chicken
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DOI：
10.1111/j.1365-2052.2010.02090.x
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发表时间：
2011-04-01
期刊：
影响因子：
2.4
通讯作者：
Kaiser, P.
中科院分区：
文献类型：
--
作者：
Fife, M. S.;Howell, J. S.;Kaiser, P.
沙门氏菌感染的家禽产品是人类沙门氏菌感染的主要来源。欧盟最近禁止在家禽生产中预防性使用抗菌剂，这增加了对控制家禽群沙门氏菌感染的替代方法的需求。对沙门氏菌定植抗性更强的鸡进行遗传选择，为可持续地控制商业家禽群中的病原体及其随后进入食物链提供了一种有吸引力的手段。对不同近交系鸡的分析表明，个体品系对已测试的沙门氏菌的许多血清型一致地敏感或抗性。在这项研究中，两个近交系的沙门氏菌定植（6（1）（R）和N（S））具有不同的敏感性，用于回交实验设计。与以前的研究，使用候选基因的方法或低密度的全基因组筛选，我们已经利用了一个高密度的标记集1255 SNP覆盖整个基因组，以确定数量性状位点（QTL）。亲本系之间的对数转换盲肠细菌水平的分析揭示了在感染后1、2、3和4天的显著差异（P < 0.05）。回交（F（1）× N）群体（n = 288）的基因型分析揭示了染色体2、3、12和25上的四个QTL，用于本研究中检查的两个性状：盲肠中对数转换的细菌计数和硬化的干酪状盲肠核心的存在。其中1个QTL位于第2染色体20 Mb处，3个QTL分别位于第3、12和25染色体96、15和1 Mb处，在全染色体水平上均达到显著水平（P < 0.05）。本研究产生的结果将为未来的育种策略提供信息，以控制商业家禽群中的这些病原体。
P>Salmonella-infected poultry products are a major source of human Salmonella infection. The prophylactic use of antimicrobials in poultry production was recently banned in the EU, increasing the need for alternative methods to control Salmonella infections in poultry flocks. Genetic selection of chickens more resistant to Salmonella colonization provides an attractive means of sustainably controlling the pathogen in commercial poultry flocks and its subsequent entry into the food chain. Analysis of different inbred chickens has shown that individual lines are consistently either susceptible or resistant to the many serovars of Salmonella that have been tested. In this study, two inbred chicken lines with differential susceptibility to Salmonella colonization (6(1)(R) and N(S)) were used in a backcross experimental design. Unlike previous studies that used a candidate gene approach or low-density genome-wide screens, we have exploited a high-density marker set of 1255 SNPs covering the whole genome to identify quantitative trait loci (QTL). Analysis of log-transformed caecal bacterial levels between the parental lines revealed a significant difference at 1, 2, 3 and 4 days post-infection (P < 0.05). Analysis of the genotypes of the backcross (F(1) x N) population (n = 288) revealed four QTL on chromosomes 2, 3, 12 and 25 for the two traits examined in this study: log-transformed bacterial counts in the caeca and presence of a hardened caseous caecal core. These included one genome-wide significant QTL on chromosome 2 at 20 Mb and three additional QTL, on chromosomes 3, 12 and 25 at 96, 15 and 1 Mb, respectively, which were significant at the chromosome-wide level (P < 0.05). The results generated in this study will inform future breeding strategies to control these pathogens in commercial poultry flocks.