IN-VITRO RADIATION SENSITIVITY OF THE LNCAP PROSTATIC TUMOR-CELL LINE
IN-VITRO RADIATION SENSITIVITY OF THE LNCAP PROSTATIC TUMOR-CELL LINE
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DOI：
10.1002/pros.2990240304
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发表时间：
1994-03-01
期刊：
影响因子：
2.8
通讯作者：
LEITH, JT
中科院分区：
文献类型：
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作者：
LEITH, JT
由于有关人前列腺癌细胞内在放射敏感性的信息极其有限，我们研究了指数生长的LNCaP细胞的体外辐射响应。由于LNCaP细胞的集落形成能力很差，用X射线照射后(0-6GY)剂量依赖的再生长曲线形式的变化来研究辐射存活。生存用单次打击多靶(SHMT)方程和线性二次(LQ)方程描述。SHMT术语中外推数(N)、准阈值剂量(D-q)和平均致死剂量(D-o)的取值和95%可信限分别为：0.9(0.7~1.0)、0.0GY和1.39(0.11)GY。LQα参数为6.80(1.13)，β参数为-0.53(2.89)。因此，LNCaP谱线的X射线剂量响应是纯指数的。在临床相关剂量(S-2)下，LNCaP系的平均存活率为51.2%。将LNCaP细胞系的S-2测定值与其他人前列腺癌细胞系(DU-145和PC-3)的测定值进行比较，发现S-2测定值的平均值为47.6%，提示人前列腺癌细胞对多种肿瘤的耐药谱可能向耐药侧偏移。(C)1994年Wiley-Liss，Inc.
Because there is extremely limited information on the intrinsic radiosensitivity of human prostatic cancer cells, we have investigated the in vitro radiation response of exponentially growing LNCaP cells. Due to the very poor colony-forming potential of the LNCaP cells, radiation survival was investigated using the dose-dependent (0-6 Gy) changes seen after X-irradiation in the shapes of regrowth curves. Survival was described using both the single-hit, multitarget (SHMT) equation and the linear-quadratic (LQ) equation. The values and 95% confidence limits of the extrapolation number (n), quasi-threshold dose (D-q), and mean lethal dose (D-o) in SHMT terminology were respectively: 0.9 (0.7-1.0), 0.0 Gy, and 1.39 (0.11) Gy. The LQ alpha and beta parameters were respectively 6.80 (1.13) and -0.53 (2.89). The X-ray dose response of the LNCaP line is, therefore, purely exponential. The mean survival at the clinically relevant dose of 2 Gy (S-2) was 51.2% for the LNCaP line. Comparison of the S-2 value for the LNCaP line with previous investigations with other human prostatic cancer cell lines (DU145 and PC-3) indicates a mean S-2 value of 47.6%, which suggests that human prostate cancer cells might he toward the resistant side of the spectrum for various classes of human neoplasms. (C) 1994 Wiley-Liss, Inc.