POTASSIUM TRANSPORT LOCI IN ESCHERICHIA-COLI K-12
POTASSIUM TRANSPORT LOCI IN ESCHERICHIA-COLI K-12
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DOI：
10.1128/jb.108.2.639-644.1971
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发表时间：
1971-01-01
影响因子：
3.2
通讯作者：
KIM, BS
中科院分区：
文献类型：
--
作者：
EPSTEIN, W;KIM, BS
需要相当高的钾浓度才能生长的大肠埃希菌突变株很容易以双重突变的形式获得，该双突变株将突变与其他五个基因座中的一个或多个突变相结合。这些基因座被称为ASTK，用于钾的运输，因为这些突变导致钾运输的变化。突变对于分离和鉴定这种类型的突变是必不可少的；在KDP+菌株中，突变的存在并不阻止菌株在含有非常低浓度的K。染色体；它们都不是非常接近任何其他基因座或kdp基因。
Mutants ofEscherichia coliK-12 requiring considerably elevated concentrations of potassium for growth are readily obtained as double mutants combining akdpmutation with a mutation in one or more of five other loci. These loci are referred to astrk, for transport of K, because these mutations result in alterations in K transport. Thekdpmutation is essential in the isolation and identification of this type of mutant; in a Kdp+strain, the presence of atrkmutation does not prevent growth of the strain in media containing very low concentrations of K. Thetrkloci are widely scattered over theE. colichromosome; none of them is very near any othertrklocus or near thekdpgenes.