Diurnal cycling of glutathione in marine phytoplankton: Field and culture studies
Diurnal cycling of glutathione in marine phytoplankton: Field and culture studies
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DOI：
10.4319/lo.2004.49.4.0991
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发表时间：
2004-07-01
影响因子：
4.5
通讯作者：
Ahnerz, BA
中科院分区：
文献类型：
--
作者：
Dupont, CL;Goepfert, TJ;Ahnerz, BA
谷胱甘肽是一种丰富的细胞内硫醇，参与光存在下产生的活性氧的解毒。我们使用短期连续培养的Emiliania huxleyi和海链藻，表明昼夜光周期导致细胞内谷胱甘肽经历至少两倍的变化，最大浓度发生在光周期。半胱氨酸浓度在E. huxleyi的浓度与此相似，最高浓度出现在夜间。在马萨诸塞州法尔茅斯盐池进行的一项补充实地研究得出了颗粒样品中谷胱甘肽浓度的类似昼夜周期。这种循环是很重要的考虑时，测量颗粒硫醇在文化和实地研究，并提供了额外的证据，谷胱甘肽在真核海洋浮游植物中起着重要的作用，作为一种抗氧化剂。
Glutathione is an abundant intracellular thiol that is involved in the detoxification of reactive oxygen species generated in the presence of light. We used short-term continuous cultures of Emiliania huxleyi and Thalassiosira pseudonana to show that a diurnal light cycle causes intracellular glutathione to undergo at least twofold variations, with maximum concentrations occurring during light periods. Cysteine concentrations also appear to vary in E. huxleyi to a similar extent, with the highest concentrations occurring at night. A complementary field study conducted at Salt Pond in Falmouth, Massachusetts, yielded a similar diurnal cycle of glutathione concentrations in particulate samples. This cycling is important to consider when measuring particulate thiols in both culture and field studies and provides additional evidence that glutathione plays an important role as an antioxidant in eukaryotic marine phytoplankton.