The feasibility of using dielectrophoresis for isolation of glioblastoma subpopulations with increased stemness
The feasibility of using dielectrophoresis for isolation of glioblastoma subpopulations with increased stemness
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DOI：
10.1002/elps.201900026
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发表时间：
2019-09-01
期刊：
影响因子：
2.9
通讯作者：
Davalos, Rafael V.
中科院分区：
文献类型：
--
作者：
Alinezhadbalalami, Nastaran;Douglas, Temple A.;Davalos, Rafael V.
癌症干细胞（CSC）是具有增加的干细胞样性质的侵袭性亚群。CSC通常对大多数标准疗法具有抗性，并且是肿瘤再增殖的原因。与正常干细胞类似，由于缺乏可靠的标记物，CSC的分离具有挑战性。基于抗原的CSC分选通常需要用多种标记物染色，这使得实验复杂、昂贵，有时还不可靠。在这里，我们研究的可行性，使用介电泳（DEP）分离胶质母细胞瘤细胞增加干细胞。我们以神经球的形式培养成胶质细胞瘤细胞系作为成胶质细胞瘤干细胞的体外模型。我们证明，球体形成细胞有较高的干细胞标记物巢蛋白的表达。接下来，我们表明，神经球的介电特性的变化，作为改变培养条件的结果。我们的研究结果表明，球状体形成细胞需要更高的电压相比，胶质母细胞瘤细胞系的正常单层培养物经历相同的DEP力的大小。本研究证实了使用DEP分离胶质母细胞瘤干细胞的可能性。
Cancer stem cells (CSCs) are aggressive subpopulations with increased stem-like properties. CSCs are usually resistant to most standard therapies and are responsible for tumor repropagation. Similar to normal stem cells, isolation of CSCs is challenging due to the lack of reliable markers. Antigen-based sorting of CSCs usually requires staining with multiple markers, making the experiments complicated, expensive, and sometimes unreliable. Here, we study the feasibility of using dielectrophoresis (DEP) for isolation of glioblastoma cells with increased stemness. We culture a glioblastoma cell line in the form of neurospheres as an in vitro model for glioblastoma stem cells. We demonstrate that spheroid forming cells have higher expression of stem cell marker, nestin. Next, we show that dielectric properties of neurospheres change as a result of changing culture conditions. Our results indicate that spheroid forming cells need higher voltages to experience the same DEP force magnitude compared to normal monolayer cultures of glioblastoma cell line. This study confirms the possibility of using DEP to isolate glioblastoma stem cells.