Optical biopsy with optical coherence tomography: Feasibility for surgical diagnostics
Optical biopsy with optical coherence tomography: Feasibility for surgical diagnostics
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DOI：
10.1006/jsre.1996.4993
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发表时间：
1997-07-15
影响因子：
2.2
通讯作者：
Fujimoto, JG
中科院分区：
文献类型：
--
作者：
Brezinski, ME;Tearney, GJ;Fujimoto, JG
背景资料：光学相干层析成像（OCT）是最近发展起来的一种紧凑型技术，它使用红外光在微米尺度上执行横截面成像。由于OCT提供的成像分辨率与传统组织学相当，并且不需要与组织表面直接接触，因此在实时手术诊断中的作用代表了逻辑扩展。在这项工作中，我们测试的可行性OCT手术诊断，通过展示显微手术干预相关的组织成像，以前未描述的观察。材料与方法：超过50个网站的神经，生殖，和微血管标本从10名患者进行了检查，死后OCT。成像后，组织登记与显微注射染料，在可见光激光的指导下，然后进行常规组织学处理，以确认身份的微观结构。结果：16 +/- 1 μ m的分辨率允许以前所未有的分辨率识别亚表面微观结构。确定的结构包括周围神经束，微血管的内弹力膜，小脑的颗粒层。结论：OCT提供组织微观结构的微米级定义的能力表明其在外科诊断中的作用。未来的体内研究值得建立其实用性与手术干预相关的发病率降低。(C)北京：科学出版社.
Background: Optical coherence tomography (OCT) is a recently developed compact technology which uses infrared light to perform cross-sectional imaging on a micrometer scale. Since OCT provides imaging at a resolution comparable to conventional histology and does not require direct contact with the tissue surface, a role in real-time surgical diagnostics represents a logical extension. In this work, we test the feasibility of OCT for surgical diagnostics by demonstrating imaging in tissue relevent to microsurgical intervention, a previously undescribed observation. Materials and methods: Over 50 sites on nervous, reproductive, and microvascular specimens from 10 patients were examined postmortem with OCT. After imaging, tissue was registered with microinjections of dye, under visible light laser guidance, followed by routine histologic processing to confirm the identity of microstructure. Results: The 16 +/- 1 mu m resolution allowed subsurface microstructure to be identified at unprecedented resolution. Structures identified included fascicles of peripheral nerves, the internal elastic membrane of microvessels, and the granular layer of the cerebellum. conclusions: The ability of OCT to provide micrometer-scale definition of tissue microstructure suggests a role in surgical diagnostics. Future in vivo investigations are merited to establish its utility for morbidity reduction associated with surgical intervention. (C) 1997 Academic Press.