Prevalence of Shiga Toxin-Producing Escherichia coli as Detected by Enzyme-Linked Immunoassays and Real-Time PCR during the Summer Months in Northern Alberta, Canada
Prevalence of Shiga Toxin-Producing Escherichia coli as Detected by Enzyme-Linked Immunoassays and Real-Time PCR during the Summer Months in Northern Alberta, Canada
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DOI：
10.1128/jcm.05211-11
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发表时间：
2011-12-01
影响因子：
9.4
通讯作者：
Kwong, Pauline
中科院分区：
文献类型：
--
作者：
Chui, Linda;Lee, Mao-Cheng;Kwong, Pauline
采用两种酶免疫分析方法和一种室内实时定量聚合酶链式反应技术，对艾伯塔省北部产志贺毒素大肠杆菌(STEC)进行了检测。在2,328份粪便样本中，8份对O157：H7 STEC呈阳性，13份对非O157 STEC呈阳性。性别(P=0.17)、年龄(P=0.81)组间差异无统计学意义。大多数阳性的腹泻粪便样本都是非血液性的。
Shiga toxin-producing Escherichia coli (STEC) in northern Alberta was detected using two enzyme immunoassays and an in-house real-time PCR. Of 2,328 stool samples, 8 were positive for O157:H7 STEC and 13 were positive for non-O157 STEC. No significant gender (P = 0.17) or age (P = 0.81) differences between groups were seen. Most positive diarrheal stool samples were nonbloody.