Different Brain Regions are Infected with Fungi in Alzheimer's Disease.
Different Brain Regions are Infected with Fungi in Alzheimer's Disease.
复制标题
DOI：
10.1038/srep15015
复制
发表时间：
2015-10-15
影响因子：
4.6
通讯作者：
Carrasco L
中科院分区：
文献类型：
--
作者：
Pisa D;Alonso R;Rábano A;Rodal I;Carrasco L
阿尔茨海默病（AD）有微生物病因的可能性已被一些研究人员提出。在这里，我们提供的证据表明，从AD患者的中枢神经系统（CNS）的组织中含有真菌细胞和菌丝。可以使用针对几种真菌的特异性抗体在细胞内和细胞外检测真菌物质。不同的大脑区域，包括外额叶皮层，小脑半球，内嗅皮层/海马和脉络丛含有真菌物质，这是不存在于对照个体的脑组织。对另外10名AD患者的脑切片分析显示，所有患者都感染了真菌。在血管中也观察到真菌感染，这可以解释AD患者中经常检测到的血管病理学。从冷冻CNS样品中提取的真菌DNA的测序鉴定了几种真菌物种。总的来说，我们的研究结果提供了令人信服的证据，在中枢神经系统中存在真菌感染的AD患者，但不是在控制个人。
The possibility that Alzheimer’s disease (AD) has a microbial aetiology has been proposed by several researchers. Here, we provide evidence that tissue from the central nervous system (CNS) of AD patients contain fungal cells and hyphae. Fungal material can be detected both intra- and extracellularly using specific antibodies against several fungi. Different brain regions including external frontal cortex, cerebellar hemisphere, entorhinal cortex/hippocampus and choroid plexus contain fungal material, which is absent in brain tissue from control individuals. Analysis of brain sections from ten additional AD patients reveals that all are infected with fungi. Fungal infection is also observed in blood vessels, which may explain the vascular pathology frequently detected in AD patients. Sequencing of fungal DNA extracted from frozen CNS samples identifies several fungal species. Collectively, our findings provide compelling evidence for the existence of fungal infection in the CNS from AD patients, but not in control individuals.