Statistics of protein library construction
Statistics of protein library construction
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DOI：
10.1093/bioinformatics/bti516
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发表时间：
2005-08-01
期刊：
影响因子：
5.8
通讯作者：
Patrick, WM
中科院分区：
文献类型：
--
作者：
Firth, AE;Patrick, WM
我们研究了与构建和采样大型蛋白质编码库相关的统计数据。使用相当简单的统计数据，我们编写了算法来估计最常用协议生成的文库的多样性，包括易错 PCR、DNA 改组、StEP PCR、寡核苷酸定向随机化、MAX 随机化、合成改组、DHR、ADO 和 SISDC。 可用性：Web 界面和 C++ 源代码可在 http://guinevere.otago.ac.nz/stats.html 联系： aef@sanger.otago.ac.nz 补充信息：上述网站上的完整数学注释、模型假设和论证、用户指南和工作示例。
We have investigated the statistics associated with constructing and sampling large protein-encoding libraries. Using fairly simple statistics we have written algorithms for estimating the diversity in libraries generated by the most commonly used protocols, including error-prone PCR, DNA shuffling, StEP PCR, oligonucleotide-directed randomization, MAX randomization, synthetic shuffling, DHR, ADO and SISDC.Availability: Web interface and C++ source code available at http://guinevere.otago.ac.nz/stats.htmlContact: aef@sanger.otago.ac.nzSupplementary information: Complete mathematical notes, model assumptions and justification, users' guide and worked examples at above website.