Detection of epistatic interactions between exotic alleles introgressed from wild barley (H. vulgare ssp. spontaneum)
Detection of epistatic interactions between exotic alleles introgressed from wild barley (H. vulgare ssp. spontaneum)
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DOI：
10.1007/s00122-010-1401-y
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发表时间：
2010-07
影响因子：
5.4
通讯作者：
M. Korff;J. Léon;K. Pillen
中科院分区：
文献类型：
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作者：
M. Korff;J. Léon;K. Pillen
数量表型的表达可以通过基因、环境以及基因与环境的互作效应来控制。此外，基因型效应可归因于主基因、数量性状座位(QTL)和基因间互作，也称为上位性互作。本研究表明双向上位性互作对与驯化相关的性状如抽穗期、株高和产量的表达具有重要作用。在以春大麦品种Scarlett为遗传背景的野生大麦导入群体S42中，抽穗期、株高和产量的标记互作效应分别为13、8和12个标记。互作座位上的显著等位基因组合在抽穗期、株高和产量上重合，表明存在多效性效应，而不是几个连锁的QTL。观察到的上位性模式的主要特征是：(1)补偿效应，即来自同一基因型的等位基因组合缓冲了表型；或(2)增强效应，即只有两个相互作用的座位上的外来等位基因的组合导致了表型的改变。本研究表明，QTL主效应的估计可能会受到与背景座位的相互作用的影响，这表明上位性效应的识别对于基因克隆和标记辅助选择是重要的。此外，本研究中检测到的基因座与候选基因之间的互作效应揭示了潜在的功能关系，这可用于进一步阐明大麦的基因网络。
The expression of a quantitative phenotype can be controlled through genotype, environment and genotype by environment interaction effects. Further, genotype effects can be attributed to major genes, quantitative trait loci (QTL) and gene by gene interactions, which are also termed epistatic interactions. The present study demonstrates that two-way epistatic interactions can play an important role for the expression of domestication-related traits like heading date, plant height and yield. In the BC2DH population S42, carrying wild barley introgressions in the genetic background of the spring barley cultivar Scarlett, 13, 8 and 12 marker by marker interaction effects could be detected for the traits heading date, plant height and yield, respectively. Significant allelic combinations at interacting loci coincided for heading date, plant height and yield suggesting the presence of pleiotropic effects rather than several linked QTL. The mode of epistasis observed was primarily characterised by either (1) compensatory effects, where allelic combinations from the same genotype buffered the phenotype, or (2) augmented effects, where only the combination of the exotic allele at both interacting loci caused an altered phenotype. The present study shows that estimates of main effects of QTL can be confounded by interactions with background loci, suggesting that the identification of epistatic effects is important for gene cloning and marker-assisted selection. Furthermore, interaction effects between loci and putative candidate genes detected in the present study reveal potential functional relationships, which can be used to further elucidate gene networks in barley.