Transcriptional regulation of RNA3 of infectious bronchitis virus.
Transcriptional regulation of RNA3 of infectious bronchitis virus.
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传染性支气管炎病毒RNA3的转录调控。
DOI：
10.1007/978-0-387-33012-9_19
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发表时间：
2006
影响因子：
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通讯作者：
Machamer,CarolynE
中科院分区：
文献类型：
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作者：
Youn,Soonjeon;Collisson,EllenW;Machamer,CarolynE
1.通过不寻常的不连续转录过程引入亚基因组RNA。1这些亚基因组RNA随后被翻译成病毒蛋白;因此，亚基因组RNA转录的调节控制了病毒复制期间病毒蛋白表达的时间和水平。冠状病毒属于巢状病毒目，根据病毒的不同产生6至9个亚基因组RNA。已建立的冠状病毒亚基因组RNA转录调控模型之一涉及称为转录调控序列（TRS）的序列。TRS在相同的病毒株中非常保守，位于每个开放阅读框的上游，并调节亚基因组RNA的产生。2 TRS与基因组5 '端前导序列的3'端也有很高的序列同源性，体与前导TRS之间的序列同源性是调控亚基因组RNA转录的重要因素。3对猪传染性胃肠炎病毒的研究表明，不仅前导序列和主体TRS之间的同源性至关重要，而且TRS侧翼序列也有助于亚基因组RNA的转录。4，5传染性支气管炎病毒（IBV）是一种组3冠状病毒，其成员感染禽类物种。IBV产生6个亚基因组RNA。尽管TRS是已知的，但TRS本身或相邻序列的重要性尚未得到解决。在这项研究中，使用感染性的cDNA克隆的IBV和靶向诱变，我们表明，IBV TRS周围的序列有助于亚基因组RNA调控。
1. INTRODUCTION of subgenomic RNAs by an unusual discontinuous transcription process. 1 These subgenomic RNAs are subsequently translated into viral proteins; regulation of subgenomic RNA transcription thus controls the timing and levels of viral protein expression during viral replication. Coronaviruses, which belong to the Nidovirales order, produce 6 to 9 subgenomic RNAs, depending on the virus. One of the established subgenomic RNA transcription regulation models for coronavirus involves sequences referred to as transcription regulation sequences (TRS). The TRS is well conserved among same strains of viruses, is located upstream of each open reading frame, and regulates subgenomic RNA production. 2 The TRS also has high sequence homology with the 3’end of the leader sequence at the 5’end of the genome, and the sequence homology between the body and leader TRS is an important factor regulating subgenomic RNA transcription. 3 Studies with transmissible gastroenteritis virus showed that not only the homology between leader and body TRS is critical, but also sequences flanking the TRS contribute to the transcription of subgenomic RNAs. 4, 5 Infectious bronchitis virus (IBV) is a Group 3 coronavirus, members of which infect avian species. IBV produces 6 subgenomic RNAs. Even though the TRS is known, importance of TRS itself or adjacent sequences have not been addressed. In this study, using an infectious cDNA clone of IBV and targeted mutagenesis, we show that the sequences surrounding the IBV TRS contribute to subgenomic RNA regulation.