Norspermidine changes the basic structure of S. mutans biofilm.
Norspermidine changes the basic structure of S. mutans biofilm.
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去甲精胺改变变形链球菌生物膜的基本结构
DOI：
10.3892/mmr.2016.5979
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发表时间：
2017-01
影响因子：
3.4
通讯作者：
Ling J
中科院分区：
文献类型：
--
作者：
Ou M;Ling J
调节变形链球菌生物膜三维结构组装的因素仍然不清楚。多胺在某些细菌的生物膜形成中是必不可少的。去甲亚精胺，一种不常见的多胺，一直是一种有争议的多胺，可以导致生物膜解体。去甲亚精胺对S.变异体生物膜仍然未知。因此，本研究探讨了去甲亚精胺对S。变形杆菌生物膜去甲亚精胺组和对照组生物膜的不同结构表明，细菌胞外多糖单元（BEU）代表了S.变形杆菌生物膜此外，去甲亚精胺对S.变形菌生物膜的形成和改变的生物膜的基本组成，这导致了一个不寻常的EPS架构。因此，5 mM去甲亚精胺通过降低细胞活力和改变生物膜结构来抑制生物膜形成。基因表达微阵列分析表明，去甲亚精胺组中不规则结构的形成可能归因于群体感应系统元件的下调（2.7-15倍）。本研究表明，BEU是S.变形菌生物膜及其组装主要由群体感应系统调节。去甲亚精胺通过影响S.变形菌群体感应系统
The factors regulating the assembly of the three-dimensional structure of Streptococcus mutans biofilms remain obscure. Polyamines are essential in biofilm formation of certain bacteria. Norspermidine, an unusual polyamine, has been a controversial polyamine that can lead to biofilm disassembly. However, the role of norspermidine in S. mutans biofilms remains unknown. Therefore, the present study investigated the impact of norspermidine on S. mutans biofilms. The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria-exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms. In addition, norspermidine inhibited S. mutans biofilm formation and changed the basic composition of the biofilm, which led to an unusual EPS architecture. Therefore, 5 mM norspermidine inhibited biofilm formation both by decreasing the rate of cell viability and changing the biofilm structure. Gene-expression microarray analysis indicated that the formation of an irregular architecture in the norspermidine group was potentially attributable to the downregulation of elements of the quorum-sensing system (by 2.7–15-fold). The present study suggested that the BEUs are a basic structure of S. mutans biofilm and its assembly is regulated majorly by the quorum-sensing system. Norspermidine can lead to structure change in BEUs by influencing S. mutans quorum-sensing system.