Cellular uptake and imaging studies of glycosylated silica nanoprobe (GSN) in human colon adenocarcinoma (HT 29 cell line).
Cellular uptake and imaging studies of glycosylated silica nanoprobe (GSN) in human colon adenocarcinoma (HT 29 cell line).
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DOI：
10.2147/ijn.s44815
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发表时间：
2013
影响因子：
8
通讯作者：
Ghalandarlaki N
中科院分区：
文献类型：
--
作者：
Mehravi B;Ahmadi M;Amanlou M;Mostaar A;Ardestani MS;Ghalandarlaki N
近年来，通过磁共振成像（MRI）的分子成像在肿瘤细胞的检测中获得了突出地位。本研究的范围是糖基化二氧化硅纳米探针（GSN）的分子成像和细胞摄取研究。本研究采用电感耦合等离子体原子发射光谱法、流式细胞仪和荧光显微镜对GSN的细胞内摄取（HT 29细胞系）进行了定量和定性分析。使用3特斯拉MRI测定该纳米颗粒的体外和体内弛豫测定法;测量GSN和Magnevist®在不同组织中的生物分布。结果表明，GSN的细胞摄取约为70%。该纳米颗粒在细胞中的r1弛豫率测量为12.9 ± 1.6 mM-1 s-1，并且基于每镧系元素钆（Gd 3+）。结果还表明每个细胞的平均细胞摄取为0.7 ± 0.009 pg Gd 3+。应该注意的是，3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四唑（MTT）测定证明细胞被有效标记而没有细胞毒性，并且使用MRI定量估计靶向造影剂的递送和摄取以及使用靶向造影剂早期检测人结肠癌细胞是可行的。这些结果表明，GSN为选择这些纳米颗粒作为纳米医学应用的适当造影剂提供了重要指导。
In recent years, molecular imaging by magnetic resonance imaging (MRI) has gained prominence in the detection of tumor cells. The scope of this study is on molecular imaging and on the cellular uptake study of a glycosylated silica nanoprobe (GSN). In this study, intracellular uptake (HT 29 cell line) of GSN was analyzed quantitatively and qualitatively with inductively coupled plasma atomic emission spectroscopy, flow cytometry, and fluorescent microscopy. In vitro and in vivo relaxometry of this nanoparticle was determined using a 3 Tesla MRI; biodistribution of GSN and Magnevist® were measured in different tissues. Results suggest that the cellular uptake of GSN was about 70%. The r1 relaxivity of this nanoparticle in the cells was measured to be 12.9 ± 1.6 mM−1 s−1 and on a per lanthanide gadolinium (Gd3+) basis. Results also indicate an average cellular uptake of 0.7 ± 0.009 pg Gd3+ per cell. It should be noted that 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay demonstrated that the cells were effectively labeled without cytotoxicity, and that using MRI for quantitative estimation of delivery and uptake of targeted contrast agents and early detection of human colon cancer cells using targeted contrast agents, is feasible. These results showed that GSN provided a critical guideline in selecting these nanoparticles as an appropriate contrast agent for nanomedicine applications.