Alteration of cartilage metabolism by cells from osteoarthritic bone
Alteration of cartilage metabolism by cells from osteoarthritic bone
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DOI：
10.1002/art.1780400713
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发表时间：
1997-07-01
影响因子：
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通讯作者：
Elson, CJ
中科院分区：
文献类型：
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作者：
Westacott, CI;Webb, GR;Elson, CJ
Objective.确定骨细胞是否改变软骨代谢。使用从9例骨关节炎（OA）患者和6例无关节炎（非关节炎[NA]）受试者的髋关节和膝关节获得的外植体建立骨细胞培养物。将NA人软骨活检样品在存在或不存在骨源性细胞的情况下孵育，并测量对软骨中糖胺聚糖（GAG）释放的影响。骨细胞培养物分泌骨钙素（OC），不含表达白细胞共同抗原的细胞。与从OA骨建立的32个培养物中的17个相比，从NA骨建立的8个培养物中没有一个显著改变软骨的GAG释放（P = 0.006）。在内侧关节损伤的膝关节中，38%来自关节内侧的培养物增加了软骨的GAG释放。相比之下，77%来自关节外侧的培养物对GAG有影响，其中38%增加和38%减少GAG释放。在OA骨细胞上清液中测量七种细胞因子。当根据其对GAG释放的影响比较上清液时，任何一种细胞因子的浓度均无显著差异。骨关节炎患者的骨细胞可以影响软骨代谢。这可能解释了为什么增加的软骨下骨活动可以预测软骨损失。
Objective. To determine whether bone cells alter cartilage metabolism.Methods. Bone cell cultures were established using explants obtained from the hip and knee joints of 9 patients with osteoarthritis (OA) and 6 subjects without arthritis (nonarthritic [NA]). NA human cartilage biopsy samples were incubated in the presence or absence of bone-derived cells, and the effects on glycosaminoglycan (GAG) release from cartilage were measured.Results. Bone cell cultures secreted osteocalcin (OC) and did not contain cells expressing leukocyte common antigen. None of the 8 cultures established from NA bone, compared with 17 of 32 from OA bone, significantly altered GAG release from cartilage (P = 0.006). In knees with medial joint damage, 38% of the cultures derived from the medial side of the joint increased GAG release from cartilage. In contrast, 77% of the cultures derived from the lateral side of the joint had an effect on GAG, with 38% increasing and 38% decreasing GAG release. Seven cytokines were measured in OA bone cell supernatants. No significant difference was apparent in the concentration of any one cytokine when supernatants were compared according to their effects on GAG release.Conclusion. Bone cells from OA patients can influence cartilage metabolism. This might explain why increased subchondral bone activity can predict cartilage loss.