Neomycin binding preserves extracellular matrix in bioprosthetic heart valves during in vitro cyclic fatigue and storage.
Neomycin binding preserves extracellular matrix in bioprosthetic heart valves during in vitro cyclic fatigue and storage.
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DOI：
10.1016/j.actbio.2008.11.004
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发表时间：
2009-05
影响因子：
9.7
通讯作者：
Vyavahare, Naren R.
中科院分区：
文献类型：
--
作者：
Raghavan, Devanathan;Starcher, Barry C.;Vyavahare, Naren R.
生物人工心脏瓣膜(BHV)尖端具有复杂的结构，由胶原、糖胺聚糖(GAG)和弹性蛋白组成的各向异性排列。戊二醛(GLUT)被用作所有临床BHV植入物的固定剂；然而，它只稳定组织中的胶原成分，其他成分如GAG和弹性蛋白在加工、储存或植入后从组织中丢失。我们以前已经证明，化学交联剂的有效性可以通过加入透明质酸酶抑制剂新霉素三硫酸盐来增加，以防止酶介导的GAG降解。在目前的研究中，我们优化了基于碳二亚胺的GAG靶向化学，在传统的GUUT交联之前将新霉素结合到BHV尖端。这种交联会增强GAG在体外循环疲劳和储存过程中的保存性。新霉素组在1000万次和5000万次加速疲劳循环后，以及在过量溶液中储存1年后，显示出更大的GAG保留率。因此，在标准的过量交联之前，将新霉素额外结合到瓣尖可以增强组织稳定性，从而增强心脏瓣膜的耐久性。
Bioprosthetic heart valve (BHV) cusps have a complex architecture consisting of an anisotropic arrangement of collagen, glycosaminoglycans (GAGs) and elastin. Glutaraldehyde (GLUT) is used as a fixative for all clinical BHV implants; however, it only stabilizes the collagen component of the tissue, and other components such as GAGs and elastin are lost from the tissue during processing, storage or after implantation. We have shown previously that the effectiveness of the chemical crosslinking can be increased by incorporating neomycin trisulfate, a hyaluronidase inhibitor, to prevent the enzyme-mediated GAG degradation. In the present study, we optimized carbodiimide-based GAG-targeted chemistry to incorporate neomycin into BHV cusps prior to conventional GLUT crosslinking. This crosslinking leads to enhanced preservation of GAGs during in vitro cyclic fatigue and storage. The neomycin group showed greater GAG retention after both 10 and 50 million accelerated-fatigue cycles and after 1 year of storage in GLUT solution. Thus, additional binding of neomycin to the cusps prior to standard GLUT crosslinking could enhance tissue stability and thus heart valve durability.
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影响因子：
4.6
作者：
SCOTT, M;VESELY, I
通讯作者：
VESELY, I
DOI：
10.1002/jbm.820210606
发表时间：
1987-06-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
NIMNI, ME;CHEUNG, D;SHEIKH, K
通讯作者：
SHEIKH, K
影响因子：
3.4
作者：
Eichinger, Walter;Daebritz, Sabine;Lange, Ruediger
通讯作者：
Lange, Ruediger
影响因子：
14
作者：
Raghavan, Devanathan;Simionescu, Dan T.;Vyavahare, Naren R.
通讯作者：
Vyavahare, Naren R.
影响因子：
4.9
作者：
Simionescu, DT;Lovekamp, JJ;Vyavahare, NR
通讯作者：
Vyavahare, NR