Dual Air-Diffusion Membrane- and Mediatorless Dihydrogen/Air-Breathing Biofuel Cell Operating at Room Temperature
Dual Air-Diffusion Membrane- and Mediatorless Dihydrogen/Air-Breathing Biofuel Cell Operating at Room Temperature
复制标题
室温下运行的双空气扩散膜和无介质氢气/呼吸空气生物燃料电池
DOI：
10.1016/j.jpowsour.2016.10.030
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发表时间：
2016
影响因子：
9.2
通讯作者：
Kenji Kano
中科院分区：
文献类型：
--
作者：
Hong-qi Xia;Keisei;So;Yuki Kitazumi;Osamu Shirai;Koji Nishikawa;Yoshiki Higuchi;Kenji Kano
以疣孢漆斑菌（Myrothecium verrucaria，BOD）的胆红素氧化酶和普通脱硫弧菌（Desulfovibrio vulgaris Miyazaki F，MBH）的膜结合[NiFe]氢化酶分别作为阴极和阳极的生物催化剂，以科琴黑改性防水碳纸（KB/WPCC）作为电极材料，构建了一种不含任何介体的无膜直接电子转移（DET）型氢/空气呼吸生物燃料电池。KB/WPCC表面分别用2-氨基苯甲酸和对苯二胺进行修饰，使BOD的带正电荷的电子接受位点和MBH的带负电荷的电子提供位点分别面对电极表面。采用气体扩散系统的电极，以实现高速基板供应。结果表明，在带负电和带正电的表面上，分别实现了BOD还原O2和MBH还原H2的电流密度的极大提高。气体扩散系统也抑制了MBH在高电极电位下的氧化失活。最后，基于改进的生物阳极和生物阴极，构建了双气体扩散膜和无介体H2/空气呼吸生物燃料电池。最大功率密度达到6.1 mW cm-2（在0.72 V），开路电压为1.12 V，使用1 atm的H2气体作为燃料，在室温和被动和静态条件下。
A membraneless direct electron transfer (DET)-type dihydrogen (H2)/air-breathing biofuel cell without any mediator was constructed wherein bilirubin oxidase fromMyrothecium verrucaria(BOD) and membrane-bound [NiFe] hydrogenase fromDesulfovibrio vulgarisMiyazaki F (MBH) were used as biocatalysts for the cathode and the anode, respectively, and Ketjen black-modified water proof carbon paper (KB/WPCC) was used as an electrode material. The KB/WPCC surface was modified with 2-aminobenzoic acid andp-phenylenediamine, respectively, to face the positively charged electron-accepting site of BOD and the negatively charged electron-donating site of MBH to the electrode surface. A gas-diffusion system was employed for the electrodes to realize high-speed substrate supply. As result, great improvement in the current density of O2 reduction with BOD and H2reduction with MBH were realized at negatively and postively charged surfaces, respectively. Gas diffusion system also suppressed the oxidative inactivation of MBH at high electrode potentials. Finally, based on the improved bioanode and biocathode, a dual gas-diffusion membrane- and mediatorless H2/air-breathing biofuel cell was constructed. The maximum power density reached 6.1 mW cm−2(at 0.72 V), and the open circuit voltage was 1.12 V using 1 atm of H2gas as a fuel at room temperature and under passive and quiescent conditions.