Transport of multiple nicotinic acetylcholine receptors in the rat optic nerve:: high densities of receptors containing α6 and β3 subunits
Transport of multiple nicotinic acetylcholine receptors in the rat optic nerve:: high densities of receptors containing α6 and β3 subunits
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DOI：
10.1111/j.1471-4159.2008.05282.x
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发表时间：
2008-06-01
影响因子：
4.7
通讯作者：
Kellar, Kenneth J.
中科院分区：
文献类型：
--
作者：
Cox, Brandon C.;Marritt, Andrea M.;Kellar, Kenneth J.
神经元烟碱型乙酰胆碱受体（nAChRs）在大鼠视网膜中含量丰富，至少有7种异聚体亚型。视网膜神经节细胞的轴突形成视神经并支配对视觉处理重要的脑区域，包括外侧膝状体核、上级丘和顶盖前核。这些投射区域中眼睛特异性层的发育取决于最初由nAChR介导的视网膜波[Feller等人，Science 272（1996），1182; Penn等人，Science 279（1998），2108; Bansal等人，神经科学杂志20（2000），7672]。大鼠单侧眼球摘除研究表明，nAChRs位于视神经轴突的末端，它们可能介导乙酰胆碱对视觉通路的影响。在这项研究中，我们使用放射性配体结合和免疫沉淀与亚基选择性抗体，以探讨在大鼠视神经nAChRs的亚基组成。我们在视神经中发现了多种nAChR亚型，所有这些亚型都含有β 2亚基。这些受体中的大多数是混合异聚体亚型，由至少三个不同的亚基组成。在这些亚型中包括已报道的大鼠CNS的任何区域的含α 6和β 3的nAChR的最高百分比和密度。
Neuronal nicotinic acetylcholine receptors (nAChRs) are abundant in the rat retina and at least seven heteromeric subtypes have been detected. Axons of retinal ganglion cells form the optic nerve and innervate areas of the brain important for visual processing, including the lateral geniculate nucleus, the superior colliculus, and the pretectal nucleus. Development of eye-specific layers in these projection areas are dependent upon retinal waves which are initially mediated by nAChRs [Feller et al., Science 272 (1996), 1182; Penn et al., Science 279 (1998), 2108; Bansal et al., J. Neurosci. 20 (2000), 7672]. Unilateral eye-enucleation studies in the rat indicate that nAChRs are on the terminals of optic nerve axons, where they may mediate influences of acetylcholine on visual pathways. In this study, we use radioligand binding and immunoprecipitation with subunit-selective antibodies to investigate the subunit composition of nAChRs in the rat optic nerve. We found multiple nAChR subtypes in the optic nerve, all of which contain the beta 2 subunit. Most of these receptors are mixed heteromeric subtypes, composed of at least three different subunits. Included among these subtypes is the highest percentage and density of alpha 6- and beta 3-containing nAChRs of any area of the rat CNS that has been reported.