Multiple origins of embryonic and tadpole myeloid cells in Xenopus laevis
Multiple origins of embryonic and tadpole myeloid cells in Xenopus laevis
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非洲爪蟾胚胎和蝌蚪骨髓细胞的多重起源
DOI：
10.1007/s00441-017-2601-4
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Mitsugu Maeno
中科院分区：
文献类型：
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作者：
Yasutaka Imai;keisuke Ishida;Maya Nemoto;Keisuke Nakata;Takashi Kato;Mitsugu Maeno
制备了非洲爪哇髓系细胞特异性过氧化物酶(MPO)兔抗血清，用于鉴定成体和幼体动物的髓系细胞。来自成人造血器官的血样涂片与MPO和XL-2共染色，XL-2是一种小鼠抗白细胞共同抗原的单抗。胸腺和脾中的淋巴细胞为XL-2+MPO−，外周血细胞和脾中的粒细胞为XL-2+MPO+，说明这两种抗体的双重染色可以对白细胞进行分类。幼虫脏器免疫组织化学分析显示，XL-2+MPO−细胞散在分布于肝脏各处，而XL-2+MPO+细胞主要分布于皮质区。有趣的是，在幼虫中肾雏形区域发现了一群XL-2+MPO+细胞。中肾区XL-2+MPO+细胞占XL-2+细胞的比例约为80%，远高于其他造血器官。为了阐明蝌蚪中肾髓系细胞的胚胎起源，进行了小鼠胚胎移植实验。LevisandX。北极熊胚胎被用来追踪X。北冰洋细胞作为供体细胞。在所观察的胚胎组织中，早期神经胚期的尾芽组织对第48期中肾区的髓样团的贡献最大。因此，在爪哇胚胎中，至少可以追溯到四个独立的髓系细胞群体的起源。
Rabbit anti-serum against a myeloid-cell-specific peroxidase (Mpo) ofXenopus laeviswas generated to identify myeloid cells in adult and larval animals. Smears of blood samples from adult hematopoietic organs were co-stained with Mpo and with XL-2, a mouse monoclonal antibody against a leukocyte common antigen. Lymphocytes found in the thymus and spleen were XL-2+Mpo−and granulocytes found in peripheral blood cells and the spleen were XL-2+Mpo+, indicating that double-staining with these two antibodies allowed classification of the leukocyte lineages. Immunohistochemical analysis of larval organs showed that XL-2+Mpo−cells were scattered throughout the liver, whereas XL-2+Mpo+cells were present mainly in the cortex region. Interestingly, a cluster of XL-2+Mpo+cells was found in the region of the larval mesonephric rudiment. The ratio of XL-2+Mpo+cells to XL-2+cells in the mesonephric region was approximately 80%, which was much higher than that found in other hematopoietic organs. In order to elucidate the embryonic origin of the myeloid cells in the tadpole mesonephros, grafting experiments betweenX. laevisandX. borealisembryos were performed to trace theX. borealiscells as donor cells. Among the embryonic tissues examined, the tailbud tissue at the early neurula stage contributed greatly to the myeloid cluster in the mesonephric region at stage 48. Therefore, at least four independent origins of the myeloid cell population can be traced in theXenopusembryo.