Arabidopsis histone demethylases LDL1 and LDL2 control primary seed dormancy by regulating DELAY OF GERMINATION 1 and ABA signaling-related genes.
Arabidopsis histone demethylases LDL1 and LDL2 control primary seed dormancy by regulating DELAY OF GERMINATION 1 and ABA signaling-related genes.
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拟南芥组蛋白去甲基化酶 LDL1 和 LDL2 通过调节 DELAY OF GERMINATION 1 和 ABA 信号相关基因控制初级种子休眠
DOI：
10.3389/fpls.2015.00159
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发表时间：
2015
影响因子：
5.6
通讯作者：
Wu K
中科院分区：
文献类型：
--
作者：
Zhao M;Yang S;Liu X;Wu K
种子休眠控制发芽，在调节植物生命周期的开始方面发挥着关键作用。种子休眠在种子成熟过程中建立并维持，并在干燥储存（后熟）过程中逐渐打破。植物激素脱落酸 (ABA) 和延迟发芽 1 (DOG1) 蛋白是种子休眠的重要调节剂。最近的研究表明，染色质修饰也参与种子休眠的转录调控。在这里，我们发现两种拟南芥组蛋白去甲基酶，赖氨酸特异性去甲基酶样 1 和 2（LDL1 和 LDL2）在抑制种子休眠方面发挥着冗余作用。 LDL1和LDL2在长角果发育早期高度表达。 ldl1 ldl2 双突变体显示种子休眠增加，而拟南芥中 LDL1 或 LDL2 的过度表达导致休眠减少。此外，我们发现 LDL1 和 LDL2 在种子休眠建立过程中抑制种子休眠相关基因的表达，包括 DOG1、ABA2 和 ABI3。此外，遗传分析表明，LDL1 和 LDL2 抑制种子休眠需要 DOG1、ABA2 和 ABI3。综上所述，我们的研究结果表明 LDL1 和 LDL2 在种子休眠中发挥着重要作用。
Seed dormancy controls germination and plays a critical role in regulating the beginning of the life cycle of plants. Seed dormancy is established and maintained during seed maturation and is gradually broken during dry storage (after-ripening). The plant hormone abscisic acid (ABA) and DELAY OF GERMINATION1 (DOG1) protein are essential regulators of seed dormancy. Recent studies revealed that chromatin modifications are also involved in the transcription regulation of seed dormancy. Here, we showed that two Arabidopsis histone demethylases, LYSINESPECIFIC DEMETHYLASE LIKE 1 and 2 (LDL1 and LDL2) act redundantly in repressing of seed dormancy. LDL1 and LDL2 are highly expressed in the early silique developing stage. The ldl1 ldl2 double mutant displays increased seed dormancy, whereas overexpression of LDL1 or LDL2 in Arabidopsis causes reduced dormancy. Furthermore, we showed that LDL1 and LDL2 repress the expression of seed dormancy-related genes, including DOG1, ABA2 and ABI3 during seed dormancy establishment. Furthermore, genetic analysis revealed that the repression of seed dormancy by LDL1 and LDL2 requires DOG1, ABA2, and ABI3. Taken together, our findings revealed that LDL1 and LDL2 play an essential role in seed dormancy.
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影响因子：
4.5
作者：
Bouyer D;Roudier F;Heese M;Andersen ED;Gey D;Nowack MK;Goodrich J;Renou JP;Grini PE;Colot V;Schnittger A
通讯作者：
Schnittger A
影响因子：
11.6
作者：
Liu, Yongxiu;Koornneef, Maarten;Soppe, Wim J. J.
通讯作者：
Soppe, Wim J. J.
影响因子：
11.4
作者：
Kushiro, T;Okamoto, M;Nambara, E
通讯作者：
Nambara, E
DOI：
10.1073/pnas.081594298
发表时间：
2001-04-10
影响因子：
11.1
作者：
Lopez-Molina, L;Mongrand, S;Chua, NH
通讯作者：
Chua, NH
影响因子：
7.4
作者：
Okamoto, M;Kuwahara, A;Nambara, E
通讯作者：
Nambara, E