Distribution of ionocytes in the saccular epithelium of the inner ear of two teleosts (Oncorhynchus mykiss and Scophthalmus maximus)

Distribution of ionocytes in the saccular epithelium of the inner ear of two teleosts (Oncorhynchus mykiss and Scophthalmus maximus)
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两种硬骨鱼(虹鳟和大菱鲆)内耳囊上皮中离子细胞的分布

DOI:
10.1007/s004410050851
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发表时间:
1997
影响因子:
3.6
通讯作者:
G. Boeuf
G. Boeuf
中科院分区:
生物学3区
文献类型:
--
作者:
N. Mayer;H. Kossmann;A. Watrin;P. Payan;G. Boeuf

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摘要:对鳟鱼(虹鳟)和大菱鲆(大菱鲆)的囊状膜进行了研究,以表征可能负责离子交换的特化上皮细胞。定位细胞类型的方法对于该组织是新的,因为使用立体显微镜和光学显微镜进行观察,以便对上皮进行总体观察。两个物种之间没有明显的差异。囊状组织是单层上皮(黄斑神经区除外),周围有一层结缔组织,周围有许多血管。使用荧光探针DAPSMI和碘化锌/锇固定染色定义了两个区域,其中存在离子细胞。在第一,大离子细胞被分组为一个几乎完整的,冠状周围的网状结构,但分开,黄斑。在第二个领域,对面的黄斑,离子细胞较小,立方体,并聚集在补丁。两个区域均存在富含Na+,K+-ATPase和碳酸酐酶II的细胞。与以前在哺乳动物和鱼类中的研究相反,在球囊的上皮中也发现了离子细胞。
Abstract.The saccular membranes of trout (Oncorhynchus mykiss) and turbot (Scophthalmus maximus) were examined to characterize specialized epithelial cells that might be responsible for ion exchange. The approach for localizing cell types was new for this tissue, as observations were made with a stereomicroscope and a light microscope in order to have a general view of the epithelium. No important differences between the two species were seen. The saccular tissue is a monolayer epithelium (except for the macula neural zone) surrounded by a layer of connective tissue invaded by many blood vessels. The use of the fluorescent probe DAPSMI and zinc iodide/osmium fixation-coloration defined two areas in which ionocytes were present. In the first, large ionocytes were grouped into a nearly complete, crowned meshwork around, but separated from, the macula. In the second area, opposite the macula, the ionocytes were smaller, cubical, and grouped in patches. Cells rich in Na+, K+-ATPase and carbonic anhydrase II were present in both areas. Contrary to previous studies in mammals and fish, ionocytes were also found in the epithelium of the saccule.
松鼠猴内耳哇巴因敏感、钾依赖性对硝基苯磷酸酶活性的超细胞化学研究。
DOI: --
发表时间: 1987
影响因子: 1.4
作者:
Yoshihara,T;Usami,S;Igarashi,M;Fermin,CD
通讯作者: Fermin,CD
DOI: 10.3109/00016489409126037
发表时间: 1994-03-01
影响因子: 1.4
作者:
ICHIMIYA, I;ADAMS, JC;KIMURA, RS
通讯作者: KIMURA, RS
DOI: 10.1016/s0196-0709(83)80005-2
发表时间: 1983-01-01
影响因子: 2.5
作者:
LIM, DJ;KARABINAS, C;TRUNE, DR
通讯作者: TRUNE, DR