Relaxin-related gene expression differs between anadromous and stream-resident stickleback (Gasterosteus aculeatus) following seawater transfer
Relaxin-related gene expression differs between anadromous and stream-resident stickleback (Gasterosteus aculeatus) following seawater transfer
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DOI：
10.1016/j.ygcen.2014.06.017
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发表时间：
2014-09-01
影响因子：
2.7
通讯作者：
Kitano, Jun
中科院分区：
文献类型：
--
作者：
Kusakabe, Makoto;Ishikawa, Asano;Kitano, Jun
松弛素（RLN）是一种激素，最初被确定为妊娠和生殖的调节因子。然而，最近的哺乳动物研究表明，松弛素也有强大的神经调节作用。在哺乳动物中，已经鉴定了六种松弛素家族肽：RLN 1/2、RLN 3、胰岛素样肽（INSL）3、IN 5L 4、INSL 5和INSL 6。以前的基因组数据库搜索显示，硬骨鱼也拥有多个松弛素家族基因。然而，这些松弛素家族肽在硬骨鱼中的功能仍不清楚。为了深入了解硬骨鱼类松弛素的神经调节功能，我们研究了广盐性三棘鱼（Gasterosteus aculeatus）的松弛素家族肽，这已经多样化成各种生态型。rln 3a，rln 3b和rln转录本在棘鱼脑中是丰富的，而insl 5 b转录本水平在肠组织中最高。海水攻击实验表明，海水转移后，脑中rln 3a，rln 3b和rln的转录水平发生显著变化。特别是，rln 3b表现出不同的模式之间的时间变化溯河产卵和流居民变形。松弛素家族肽受体rxfp 1，rxfp 2b，rxfp 3 -2a和rxfp 3 -2b的转录水平在大脑中没有表现出实质性的变化，尽管这些在溯河产卵的变体中总是高于溪流驻留的变体。这些结果表明，刺鱼松弛素系统的差异调节盐度信号，至少在转录水平上，和溯河产卵和流居民形态不同松弛素信号通路。松弛素相关基因在这两种形态之间表达的差异为进一步研究松弛素在硬骨鱼类中的生理调节功能奠定了基础。(C)2014爱思唯尔公司All rights reserved.
Relaxin (RLN) is a hormone that was originally identified as a regulator of pregnancy and reproduction. However, recent mammalian studies have demonstrated that relaxins also have potent osmoregulatory actions. In mammals, six relaxin family peptides have been identified: RLN1/2, RLN3, insulin-like peptide (INSL) 3, IN5L4, INSL5, and INSL6. Previous genome database searches have revealed that teleosts also possess multiple relaxin family genes. However, the functions of these relaxin family peptides in teleosts remain unclear. In order to gain insight into the osmoregulatory functions of teleost relaxins, we studied the relaxin family peptides in euryhaline three-spined sticklebacks (Gasterosteus aculeatus), which have diversified into a variety of ecotypes. Rln3a, rln3b, and rln transcripts were abundant in the stickleback brain, whereas insl5b transcript levels were highest in the intestine among tissues. Seawater challenge experiments showed that transcript levels of rln3a, rln3b, and rln in the brain changed significantly after seawater transfer. Particularly, rln3b showed different patterns of temporal changes between anadromous and stream-resident morphs. The transcript levels of relaxin family peptide receptors, rxfp1, rxfp2b, rxfp3-2a, and rxfp3-2b, did not exhibit substantial changes in the brain, although these were constantly higher in the anadromous morph than the stream-resident morph. These results suggest that stickleback relaxin systems are differentially regulated by salinity signals, at least at the transcriptional level, and anadromous and stream-resident morphs differ in relaxin signaling pathways. The differences in the expression of relaxin-related genes between these two morphs provide a foundation for further exploration of the osmoregulatory function of relaxins in teleosts. (C) 2014 Elsevier Inc. All rights reserved.