AtSPX1-mediated transcriptional regulation during leaf senescence in Arabidopsis thaliana
AtSPX1-mediated transcriptional regulation during leaf senescence in Arabidopsis thaliana
复制标题
AtSPX1 介导的拟南芥叶片衰老过程中的转录调控
DOI：
10.1016/j.plantsci.2019.03.005
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发表时间：
2019
期刊：
影响因子：
5.2
通讯作者：
Su Zhen
中科院分区：
文献类型：
--
作者：
Yan Hengyu;Sheng Minghao;Wang Chunchao;Liu Yue;Yang Jiaotong;Liu Fengxia;Xu Wenying;Su Zhen
叶片衰老是叶片生长的最后阶段，是一个高度协调的复杂过程。磷作为植物生长所必需的大量营养素，从衰老的叶片再转移到植物的其他旺盛部分。本研究通过数据挖掘，发现一些磷饥饿诱导的基因如AtSPX 1在拟南芥衰老叶片中受到显著诱导。结果表明，AtSPX 1基因的过表达可加速叶片衰老，抑制Pi的积累，提高叶片中SA的产生和H2 O2的含量，而AtSPX 1基因的突变株则表现出轻微的延缓叶片衰老的特性。我们进行了基于RNA-seq的转录组分析以及转基因与野生型植物的GO和GSEA富集分析，以阐明可能的潜在调控机制。在过量表达植株35 S：：AtSPX 1/WT中表达上调的558个基因在叶片衰老、磷饥饿反应和SA信号转导途径中显著富集，同时也是WRKYs和NAC等转录因子的靶基因。综上所述，AtSPX 1在拟南芥叶片衰老、磷饥饿和SA信号通路之间起着重要的调控作用。
Leaf senescence is the final stage of leaf growth, a highly coordinated and complicated process. Phosphorus as an essential macronutrient for plant growth is remobilized from senescing leaves to other vigorous parts of the plant. In this study, through data mining, we found some phosphate starvation induced genes such asAtSPX1, were significantly induced in aging leaves in Arabidopsis. We applied a reverse genetics approach to investigate the phenotypes of transgenic plants and mutant plants, and the results showed that the overexpression ofAtSPX1accelerated leaf senescence, suppressed Pi accumulation, promoted SA production and H2O2levels in leaves, while the mutant lines ofAtSPX1showed slightly delayed leaf senescence. We conducted RNA-seq-based transcriptome analysis together with GO and GSEA enrichment analyses for transgenic vs. wild-type plants to elucidate the possible underlying regulatory mechanism. The 558 genes that were up-regulated in the overexpression plants35S::AtSPX1/WT,were significantly enriched in the process of leaf senescence, Pi starvation responses and SA signaling pathways, as were the target genes of some transcription factors such as WRKYs and NACs. In a word, we characterized AtSPX1 as a key regulator, which mediated the crosstalks among leaf senescence, Pi starvation and SA signaling pathways inArabidopsis thaliana.