Ion-Switchable Quantum Dot Förster Resonance Energy Transfer Rates in Ratiometric Potassium Sensors.
Ion-Switchable Quantum Dot Förster Resonance Energy Transfer Rates in Ratiometric Potassium Sensors.
复制标题
离子切换的量子点förster共振能量转移速率中的钾传感器。
DOI：
10.1021/acsnano.5b05396
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发表时间：
2016-04-26
期刊：
影响因子：
17.1
通讯作者：
Clark HA
中科院分区：
文献类型：
--
作者：
Ruckh TT;Skipwith CG;Chang W;Senko AW;Bulovic V;Anikeeva PO;Clark HA
用于实时光学成像细胞钾浓度的工具目前仅限于一小组分子指示剂染料。基于量子点的纳米传感器比有机指示剂具有更高的光稳定性和可调谐性，但之前的设计在尺寸、灵敏度和选择性方面存在不足。在这里，我们介绍一种用于钾测量的小型、灵敏、选择性纳米传感器。动态猝灭剂调节两种不同量子点种类发射的荧光以产生比率信号。我们表征了钾调制传感器的特性并研究了传感器内的光子相互作用。猝灭剂的质子化会随着钾的变化而变化，从而改变其福斯特辐射能量转移率以及与每个量子点物种相应的相互作用半径。纳米传感器对细胞环境中典型的钾浓度变化做出响应，从而为生物事件期间的钾通量成像提供了一种有前景的工具。
The tools for optically imaging cellular potassium concentrations in real-time are currently limited to a small set of molecular indicator dyes. Quantum dot-based nanosensors are more photostable and tunable than organic indicators, but previous designs have fallen short in size, sensitivity, and selectivity. Here we introduce a small, sensitive, and selective nanosensor for potassium measurements. A dynamic quencher modulates the fluorescence emitted by two different quantum dot species to produce a ratiometric signal. We characterized the potassium-modulated sensor properties and investigated the photonic interactions within the sensors. The quencher’s protonation changes in response to potassium, which changes its Förster radiative energy transfer rate and the corresponding interaction radii with each quantum dot species. The nanosensors respond to changes in potassium concentrations typical of the cellular environment, and thus provide a promising tool for imaging potassium fluxes during biological events.