Superparamagnetic nanoparticles for the treatment of periodontal disease
Superparamagnetic nanoparticles for the treatment of periodontal disease
复制标题
超顺磁性纳米颗粒治疗牙周病
DOI：
10.1117/12.2654656
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Xu, Wei
中科院分区：
文献类型：
--
作者：
Fritz, Shayden;Armijo, Leisha M.;Simpson, Kayla;Lopez, Kimberly;Osinski, Marek;Bandara, Nihal;Smyth, Hugh D.;Sheng, Jian;Xu, Wei
牙周病在世界范围内普遍存在，并且由于生态失调和慢性炎症状态与许多其他健康状况有关。大多数牙周病是由致病菌引起的，这些致病菌以生物膜的形式定植在牙齿组织中。由于牙齿和牙龈的结构复杂，清除细菌生物膜很难实现。由于直接接触牙刷刷毛、牙线和磨料牙膏的机械破坏是有限的，因此正畸金属丝和牙科设备给传统方法充分去除生物膜带来了额外的障碍。磁活化纳米颗粒（NPs），特别是氧化铁纳米颗粒（IONPs），可以被功能化为抗菌颗粒，并通过磁场远程控制，是口腔生物膜根除的兴趣。我们提供了多物种细菌培养、已建立的生物膜、人类牙龈角质形成细胞和单独的人类牙龈成纤维细胞以及多物种生物膜共同培养的数据，以确定去除牙齿生物膜的最安全、最有效的IONP大小范围和活性磁性NPs的处理浓度。我们报告了海藻酸盐包被的IONPs比葡聚糖包被的IONPs更有效，并且小尺寸（~ 8nm比bbb20 nm）似乎表现出更强的抗菌效果。人牙龈角质细胞（TIGK）细胞与处理过的和未处理过的多物种生物膜共同培养，在抗菌牙周炎模型中，ionp处理过的生物膜也显示出炎症标志物减少的趋势。
Periodontal diseases are prevalent worldwide and are linked to numerous other health conditions due to dysbiosis and chronic inflammatory state. Most periodontal diseases are caused by pathogenic bacteria that colonize dental tissues in the form of biofilm. Eradication of bacterial biofilms can be difficult to achieve due to the complex architecture of the teeth and gums which complicates the removal. Orthodontic wires and dental devices introduce additional hurdles to the adequate removal of biofilms by traditional methods since mechanical disruption via direct contact with toothbrush bristles, floss, and abrasive toothpaste is limited. Magnetically activated nanoparticles (NPs), specifically iron oxide nanoparticles (IONPs) that can be functionalized as antimicrobial particles and remotely controlled by magnetic fields, are of interest for oral biofilm eradication. We present data in multi-species bacterial cultures, established biofilms, human gingival keratinocytes, and human gingival fibroblast cells alone and in the presence of multispecies biofilm co-cultures to determine the safest, most efficacious IONP size ranges and treatment concentrations of active magnetic NPs for removal of dental biofilms. We report enhanced efficacy for IONPs coated with alginate vs. dextran, and small sizes (~8 nm vs. >20 nm in size) appear to exhibit enhanced antimicrobial efficacy. Human gingival keratinocyte (TIGK) cells in co-culture with treated and untreated multispecies biofilms in anin-vitroperiodontitis model also exhibited a trend of reduced inflammatory markers in wells with IONP-treated biofilms.
氧化铁纳米颗粒和磁场对口腔生物膜的影响
影响因子：
--
作者：
Gema J. Alas;Ronald E. Pagano;Jane Q. Nguyen;H. Bandara;S. Ivanov;G. Smolyakov;D. Huber;H. Smyth;M. Osiński
通讯作者：
M. Osiński
影响因子：
3.7
作者：
Benov, Ludmil
通讯作者：
Benov, Ludmil
影响因子：
4.4
作者：
Brookes ZLS;Bescos R;Belfield LA;Ali K;Roberts A
通讯作者：
Roberts A