First integrative study of the diversity and specificity of metacercariae of Posthodiplostomum Dubois, 1936 from native and introduced fishes in the Caribbean
First integrative study of the diversity and specificity of metacercariae of Posthodiplostomum Dubois, 1936 from native and introduced fishes in the Caribbean
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DOI：
10.1017/s0031182022001214
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发表时间：
2022-08
期刊：
影响因子：
2.4
通讯作者：
Sandra C. Díaz Pernett;S. Brant;S. Locke
中科院分区：
文献类型：
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作者：
Sandra C. Díaz Pernett;S. Brant;S. Locke
摘要 摘要 淡水鱼宿主中经常发现后双口属的囊蚴。虽然该属的多样性和分类学在分子系统发育研究中受到越来越多的关注，但现有数据仍然存在地理偏差。大多数对后双口线虫和形态相似（neascus）蠕虫的分子研究起源于北美、欧洲和亚洲（超过 60% 的 DNA 序列来自美国和加拿大），目前来自新热带地区的数据很少，那里的宿主多样性较高，表明寄生虫多样性较高且采样不足。在这项研究中，我们报告了波多黎各鱼类中后双口线虫囊蚴的分子和形态学数据，此前该地区仅报道过一个物种。来自 Dajaus monticola（原产于波多黎各）囊蚴和引进鱼类 Poecilia reticulata、Parachromis managuensis、Lepomis Macrochirus 和 Micropterus salmoides 的细胞色素 C 氧化酶亚基 1 的部分序列揭示了 7 个遗传上不同的物种水平谱系，其中 4 个是新的。我们报告了Posthodiplostomum macrocotyle（丽鱼科鱼Pa. managuensis肌肉中的囊蚴）中新的分子生命周期联系，这种物种以前仅在南美鸟类的成虫中被发现。和 Posthodiplostomum sp。 23（poeciliids 中的囊蚴），最近在美国佐治亚州的 Ardea Herodias 中发现。我们还报告了 Posthodiplostomum sp 的第一个分子数据。加勒比海地区的大鲑鱼中有 8 种。大多数物种的囊蚴在形态上是不同的，并且都对鱼类宿主表现出狭窄的特异性，没有迹象表明引进鱼类和本地鱼类之间存在共享寄生虫。
Abstract Abstract Metacercariae of the genus Posthodiplostomum are often recorded in freshwater fish hosts. While the diversity and taxonomy of this genus are receiving increasing attention in molecular phylogenetic studies, available data remain geographically biased. Most molecular studies of Posthodiplostomum and morphologically similar (neascus) worms originate in North America and Europe and Asia (more than 60% of DNA sequences are from USA and Canada), with few data currently available from the Neotropics, where high host diversity suggests high and under-sampled parasite diversity. In this study, we report molecular and morphological data from metacercariae of Posthodiplostomum in fish in Puerto Rico, where only a single species has been previously reported. Partial sequences of cytochrome c oxidase subunit 1 from metacercariae from Dajaus monticola (native to Puerto Rico) and the introduced fishes Poecilia reticulata, Parachromis managuensis, Lepomis macrochirus and Micropterus salmoides revealed 7 genetically distinct species-level lineages, of which 4 were novel. We report novel molecular life-cycle linkages in Posthodiplostomum macrocotyle (metacercariae in muscle of the cichlid Pa. managuensis), a species previously known only from adults in birds from South America; and in Posthodiplostomum sp. 23 (metacercariae in poeciliids), which has recently been found in Ardea herodias in Georgia, USA. We also report the first molecular data from Posthodiplostomum sp. 8 in M. salmoides in the Caribbean. Metacercariae of most species were morphologically distinguished and all displayed narrow specificity for fish hosts, with no indication of parasite sharing among introduced and native fishes.