Liquid droplet formation and cytoplasm to vacuole targeting of aminopeptidase I are temperature sensitive in Saccharomyces cerevisiae
Liquid droplet formation and cytoplasm to vacuole targeting of aminopeptidase I are temperature sensitive in Saccharomyces cerevisiae
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DOI：
10.1002/1873-3468.14509
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发表时间：
2022-10-19
期刊：
影响因子：
3.5
通讯作者：
Hirata，Eri
中科院分区：
文献类型：
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作者：
Suzuki，Kuninori;Hirata，Eri
氨基肽酶 I (Ape1) 是酿酒酵母细胞质到液泡靶向 (Cvt) 途径的主要货物之一，这是一种选择性自噬。合成后，Ape1 前体 (prApe1) 经过相分离，在细胞质中形成液滴，称为 Ape1 液滴。在这项研究中，我们发现表达 prApe1-GFP 的细胞表现出对温度敏感的 Ape1 液滴形成，这影响了其运输。此外，我们还发现，由于在高温下 Ape1 液滴形成存在缺陷，内源性 Ape1 转运在各种实验室菌株中存在缺陷。最后，我们发现表现出耐热生长的基因破坏子抑制了 Ape1 运输的温度敏感性。 Ape1 液滴的形成可能是高温下细胞质完整性的指标。
Aminopeptidase I (Ape1) is one of the major cargoes of the cytoplasm‐to‐vacuole targeting (Cvt) pathway, which is a kind of selective autophagy, inSaccharomyces cerevisiae. After synthesis, the Ape1 precursor (prApe1) undergoes phase separation to form liquid droplets, termed Ape1 droplets, in the cytoplasm. In this study, we found that cells expressing prApe1‐GFP exhibited temperature‐sensitive formation of Ape1 droplets, which affected its transport. Moreover, we showed that endogenous Ape1 transport was defective at high temperatures in various laboratory strains due to the defect in the formation of Ape1 droplets at these temperatures. Finally, we found that gene disruptants showing heat‐tolerant growth suppressed the temperature sensitivity of the Ape1 transport. The formation of Ape1 droplets might be an indicator of cytoplasmic integrity at high temperature.