Framework of the enteric nerve plexuses: an immunocytochemical study in the guinea pig jejunum using an antiserum to S-100 protein.
Framework of the enteric nerve plexuses: an immunocytochemical study in the guinea pig jejunum using an antiserum to S-100 protein.
复制标题
肠神经丛的框架：使用 S-100 蛋白抗血清在豚鼠空肠中进行的免疫细胞化学研究。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
E. Daniel
中科院分区：
文献类型：
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作者：
S. Kobayashi;M. Suzuki;T. Endo;S. Tsuji;E. Daniel
用改良的过氧化物酶-抗过氧化物酶法，用抗S-100蛋白的抗血清，对豚鼠空肠各层的免疫染色切片和整体标本进行了研究。S-100蛋白免疫阳性神经胶质细胞呈精细的网格状分布于纵肌层、肌间神经丛或奥尔巴赫神经丛、包括深肌丛在内的环肌层、包括粘膜下神经丛或迈斯纳神经丛在内的粘膜下层、粘膜肌层和粘膜固有层。整个肠神经丛由两个子系统组成：肌层神经丛和粘膜下及粘膜层神经丛。这两个子系统通过穿过内环肌层的粗的连接分支彼此连接。外源性神经进入肌间神经丛形成了一个专门的交界结构，含有S-100蛋白免疫阳性胶质细胞，而那些进入粘膜下神经丛运行沿着粘膜下动脉。我们建议用肠神经胶质细胞来表示包裹肠神经元胞体和突起的S-100蛋白免疫阳性细胞。肠神经丛中从最大的神经节到最细的神经束的所有结构的框架都由这些肠胶质细胞构成。肠神经胶质细胞的光谱。肌间神经节和粘膜下神经节的星形胶质细胞与中枢神经系统的星形胶质细胞和周围神经节的卫星细胞相似。肌间神经丛初级束和次级束内的神经元与神经突起共同构成一种神经纤维。位于肌被第三束的神经束构成了自主神经丛的骨架。我们初步得出结论，Cajal间质细胞含有S-100蛋白的免疫反应性，因此在性质上是胶质细胞。发生专门的肠神经胶质细胞的神经元样功能进行了讨论，在自主神经丛的肌被。在粘膜固有层，有一个良好的网格S-100蛋白免疫阳性的肠胶质细胞。这种格子结构对应于绒毛丛和腺周丛中的Cajal间质细胞。
Immunostained sections and whole-mount preparations of the layers of the guinea pig jejunum were investigated by an improved peroxidase-antiperoxidase method using an antiserum to S-100 protein. A delicate latticework of S-100 protein immunopositive glial cells was demonstrated extending in the longitudinal muscle layer, myenteric or Auerbach's plexus, circular muscle layer including the deep muscular plexus, submucous layer including the submucous or Meissner's plexus, lamina muscularis mucosae and lamina propria mucosae. The whole enteric nerve plexuses consisted of two subsystems; nerve plexuses of the muscular coat and those of the submucous and mucous coats. These two subsystems were joined to each other by thick, connecting branches perforating the inner circular muscle layer. Extrinsic nerves entering the myenteric plexus formed a specialized junctional structure containing S-100 protein immunopositive glial cells, whereas those entering the submucous plexus ran along the submucous arteries. We proposed the term enteroglial cells to designate the S-100 protein immunopositive cells which ensheathed the somata and processes of the enteric neurons. The frameworks of all structures in the enteric nerve plexuses from the largest ganglia to the thinnest nerve fasciculi were constructed of these enteroglial cells. A spectrum of the enteroglial cells was presented. Those in the myenteric and submucous ganglia were found similar to the astroglia of the central nervous system and to the satellite cells in the peripheral ganglia. Those in the primary and secondary fasciculi of the myenteric plexus formed a kind of neuropil together with the neuronal processes. Those in the tertiary fasciculi of the muscular coat formed the framework of the autonomic ground plexus. We tentatively concluded that the interstitial cells of Cajal contain an immunoreactivity for S-100 protein, and thus are glial in nature. The occurrence of specialized enteroglial cells with a neuron-like function was discussed in the autonomic ground plexus of the muscular coat. In the lamina propria mucosae, there was a fine latticework of the S-100 protein immunopositive enteroglial cells. This latticework corresponded to that of the interstitial cells of Cajal in the villous and periglandular plexuses.