Adsorption characteristics of bovine serum albumin onto alumina with a specific crystalline structure
Adsorption characteristics of bovine serum albumin onto alumina with a specific crystalline structure
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DOI：
10.1007/s10856-013-5086-z
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发表时间：
2014-02
期刊：
影响因子：
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通讯作者：
M. Kawashita;Junpei Hayashi;Zhixia Li;T. Miyazaki;M. Hashimoto;Hiroki Hihara;H. Kanetaka
中科院分区：
文献类型：
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作者：
M. Kawashita;Junpei Hayashi;Zhixia Li;T. Miyazaki;M. Hashimoto;Hiroki Hihara;H. Kanetaka
含有具有特定晶体结构的氧化铝颗粒的骨水泥显示出与骨结合的能力。这些颗粒(Al-P)主要由三角型氧化铝(δ-Al_2O_3)组成。人体环境中存在的一些蛋白质很可能被吸附在骨水泥上，影响其生物活性的表达。然而，这种蛋白质的吸附对骨水泥的骨结合机制的影响还没有被阐明。在本研究中，我们研究了AL-P对牛血清白蛋白的吸附特性，并将其与同样具有骨结合能力的羟基磷灰石(HA)以及不与骨结合的α-型氧化铝(α-Al_2O_3)的吸附特性进行了比较。牛血清白蛋白在AL-P上的吸附特性与α-Al_2O_3有很大不同，但与HA的吸附特性非常相似。推测牛血清白蛋白是通过离子间相互作用吸附在Al-P和HA上的，而在α-Al_2O_3上是通过静电吸引吸附的。结果提示，白蛋白在种植体材料上的特异性吸附可能在材料的骨结合能力的表达中起作用。
Bone cement containing alumina particles with a specific crystalline structure exhibits the ability to bond with bone. These particles (AL-P) are mainly composed of delta-type alumina (δ-Al2O3). It is likely that some of the proteins present in the body environment are adsorbed onto the cement and influence the expression of its bioactivity. However, the effect that this adsorption of proteins has on the bone-bonding mechanism of bone cement has not yet been elucidated. In this study, we investigated the characteristics of the adsorption of bovine serum albumin (BSA) onto AL-P and compared them with those of its adsorption onto hydroxyapatite (HA), which also exhibits bone-bonding ability, as well as with those of adsorption onto alpha-type alumina (α-Al2O3), which does not bond with bone. The adsorption characteristics of BSA onto AL-P were very different from those onto α-Al2O3but quite similar to those onto HA. It is speculated that BSA is adsorbed onto AL-P and HA by interionic interactions, while it is adsorbed onto α-Al2O3by electrostatic attraction. The results suggest that the specific adsorption of albumin onto implant materials might play a role in the expression of the bone-bonding abilities of the materials.