The effect of denervation on liver regeneration in partially hepatectomized rats
The effect of denervation on liver regeneration in partially hepatectomized rats
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DOI：
10.1016/j.jss.2007.01.007
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发表时间：
2007-09-01
影响因子：
2.2
通讯作者：
Kanematsu, Takashi
中科院分区：
文献类型：
--
作者：
Hamada, Takayuki;Eguchi, Susumu;Kanematsu, Takashi
背景/目的。在部分肝移植中，在活跃的肝再生过程中，解剖的肝神经没有被修复。事实上，这种肝去神经对肝再生的病理生理影响还没有完全阐明。本研究的目的是阐明全肝失神经对肝再生的影响。方法：研究方法。实验一：测定去神经大鼠(n=5)和假手术大鼠(n=5)肝脏去甲肾上腺素的含量，以证实去神经对大鼠肝脏的影响。同时测定去神经大鼠(n=5)和假手术大鼠(n=5)肝脏微循环的变化。实验2：大鼠(n=80)随机分为两组：糖尿病肾病组(n=40)：去肝神经后肝部分切除(PH)。对照组(n=40)：假手术+PH组。两组动物分别于PH后12、24、36、48、72、120、168 h处死。测定各时间点大鼠肝脏/体重比和增殖细胞核抗原(PCNA)标记指数。结果。实验1：在糖尿病肾病大鼠体内，去甲肾上腺素几乎完全耗尽(99%)。此外，糖尿病肾病大鼠肝组织血流量明显增加。实验二：在168 h时，糖尿病肾病组大鼠的肝体比也显著高于对照组(P<；0.05)。对照组的增殖细胞核抗原标记指数在24~36h达到高峰，而糖尿病肾病组为延迟高峰。72h和120h，糖尿病肾病组的增殖细胞核抗原标记指数显著高于对照组(P<0.05)。结论。因此，在肝部分切除后，发现完全去神经可以促进肝再生。这种现象部分是由于流向残肝的肝脏血流量增加所致。(C)2007 Elsevier Inc.保留所有权利。
Background/Aim. In a partial liver transplantation, the dissected hepatic nerves are left unrepaired during active liver regeneration. In fact, the pathophysiological influence of such hepatic denervation on liver regeneration has not yet been fully clarified. The aim of the present study is to elucidate the effect of total hepatic denervation on liver regeneration. Methods. Experiment 1: To confirm the effect of hepatic denervation, the hepatic contents of norepinephrine were measured in both denervated (n = 5) and sham (n = 5) rats. The changes in the hepatic microcirculation were also measured in both denervated (n = 5) and sham (n = 5) rats. Experiment 2: The rats (n = 80) were randomly assigned to two groups: DN group (n = 40); hepatic denervation followed by a partial hepatectomy (PH). Control group (n = 40); sham hepatic denervation followed by PH. In both groups, the animals were killed at 12, 24, 36, 48, 72, 120, and 168 h after PH, respectively. The liver to body weight ratio and the proliferating cell nuclear antigen (PCNA) labeling index were measured at each time point. Results. Experiment 1: Nearly a total depletion of norepinephrine (< 99%) was observed in the DN rats. In addition, the hepatic tissue blood flow significantly increased in the DN rats. Experiment 2: The liver to body weight ratio of the DN group was also significantly higher than that of the control group at 168 h (P < 0.05). The PCNA labeling index peaked between 24 and 36 h in the control group, while that in the DN group showed a delayed peak. At 72 and 120 h, the PCNA labeling index was significantly higher in the DN group than in the control group (P < 0.05). Conclusion. Total hepatic denervation was thus found to enhance liver regeneration after a partial hepatectomy. This phenomenon is partially triggered by the increased hepatic blood flow to the remnant liver. (c) 2007 Elsevier Inc. All rights reserved.