THE ORIGIN OF THE INTRINSIC GLYCOPROTEINS OF THE RABBIT VITREOUS BODY - AN IMMUNOHISTOCHEMICAL AND AUTORADIOGRAPHIC STUDY
THE ORIGIN OF THE INTRINSIC GLYCOPROTEINS OF THE RABBIT VITREOUS BODY - AN IMMUNOHISTOCHEMICAL AND AUTORADIOGRAPHIC STUDY
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DOI：
10.1016/0014-4835(90)90045-v
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发表时间：
1990-05-01
影响因子：
3.4
通讯作者：
PELLETIER, G
中科院分区：
文献类型：
--
作者：
HADDAD, A;DEALMEIDA, JC;PELLETIER, G
软骨基质糖蛋白（CMGP）以前在人和牛的玻璃体中发现，现在通过免疫组织化学技术在1-22月龄兔的玻璃体中发现。纤毛上皮内层上皮细胞含有与CMGP特异性抗体具有免疫交叉反应性的物质。玻璃体内注射[3H]病灶后，通过放射自显影测定，这些细胞也分泌糖蛋白。在6月龄和22月龄家兔玻璃体和睫状体的sds -聚丙烯酰胺凝胶的荧光图中，可以鉴定出大约14个带，代表含有病灶残基的内在糖蛋白。玻璃体内注射[3H]病灶后，玻璃体和睫状体样品的凝胶在几个时间点的荧光图显示至少有7个共同的蛋白带，并显示标记的睫状体糖蛋白的周转。这些结果表明，纤毛上皮的内层是玻璃体糖蛋白的来源，这些糖蛋白可能在动物的整个生命周期中不断更新。
A cartilage matrix glycoprotein (CMGP), previously identified in human and bovine vitrous, now has been found in the vitreous body of rabbits aged 1-22 months by immunohistochemical techniques. Epithelial cells of the inner layer of the ciliary epithelium contain material that has immunologic cross-reactivity with a specific antibody to CMGP. These cells also secrete glycoproteins, as determined by autoadiography after intravitreal injection of [3H]fucose. Approximately 14 bands, representing intrinsic glycoproteins containing fucose residues, can be identified in fluorograms of SDS-polyacrylamide gels of samples of vitreous and ciliary bodies from 6- and 22-month-old rabbits. Fluorograms of gels of samples of vitreous and ciliary bodies from several time points after intravitreal injection of [3H]fucose reveal at least seven comigrating protein bands and also demonstrate turnover of the labeled ciliary body glycoproteins. These results suggest that the inner layer of the cililary epithelium is the source of the glycoproteins of the vitreous body and that these glycoproteins undergo turnover, probably throughout the entire life of the animals.