A Yeast DNA Microarray for the Evaluation of Toxicity in Environmental Water Containing Burned Ash
A Yeast DNA Microarray for the Evaluation of Toxicity in Environmental Water Containing Burned Ash
复制标题
用于评估含有烧灰的环境水中的毒性的酵母 DNA 微阵列
DOI：
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发表时间：
2004
影响因子：
3
通讯作者：
H. Iwahashi
中科院分区：
文献类型：
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作者：
H. J. Kim;E. Ishidou;E. Kitagawa;Y. Momose;H. Iwahashi
关于燃烧灰的危害评估和流行病学健康反应的大量研究已被报道。然而，关于燃烧灰烬中未知化学复合物的潜在毒性的信息很少。为了全面评估烧灰的多重毒性，本研究中使用了DNA微阵列，作为评估这些毒性的新尝试。本研究利用酵母DNA芯片上的全局基因表达来反映mRNA水平的变化，发现了许多细胞稳态和应激反应等作用的证据，以对抗对酵母细胞的毒性作用。在5117个开放阅读框（orf）基因中，997个基因上调，1259个基因下调，2861个基因保持不变。微阵列的详细分析揭示了与亚细胞定位、能量/代谢、各种应激反应/细胞稳态和解毒功能动态相关的基因。值得注意的是，在燃烧的灰烬中，活性氧（ROS）、金属和其他异种生物引起的毒性显示出来。同时，根据DNA修复相关基因，提出了烧灰具有致突变性的可能性。
Numerous studies on the hazard assessment and epidemiological healthresponses to burned ash have been reported. However, there is littleinformation on the potential toxicity of unknown chemical complexesin burned ash. For an overall evaluation of the multiple toxicitiesof burned ash, a DNA microarray was used in this study, as a new attempt to assess these toxicities. Using the global gene expression on yeast DNA chip to reflect the changes in mRNA levels, our study discovered a lot of evidences for the action of cell homeostasis and stress response etc., against the toxic effects on yeast cells. On the genes of 5,117 open reading frames(ORFs), as valid spots in a microarray, 997 were up-regulated, 1,259 were down-regulated and 2,861 remained unchanged. A detailed analysis of the microarray revealed the genes that were dynamically correlated to the function of the subcellular localization, energy/metabolism, various stress responses/cell homeostasis and detoxification. Significantly, the toxicities, caused by reactive oxygen species(ROS), metals and the other xenobiotics, were indicated in burned ash. Also, the possibility of mutagenicity of the burned ash was suggested on the basis of the DNA repair related genes.
酿酒酵母 MRT4、GRC5、SLA2 和 THS1 基因中的温度敏感突变会导致 mRNA 周转缺陷。
影响因子：
3.3
作者：
Zuk,D;Belk,JP;Jacobson,A
通讯作者：
Jacobson,A