Effect of essential fatty acid deficiency on cutaneous sterol synthesis.
Effect of essential fatty acid deficiency on cutaneous sterol synthesis.
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必需脂肪酸缺乏对皮肤甾醇合成的影响。
DOI：
10.1111/1523-1747.ep12455835
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Elias,PM
中科院分区：
文献类型：
--
作者：
Feingold,KR;Brown,BE;Lear,SR;Moser,AH;Elias,PM
皮肤是全身甾体生成的主要部位，再加上角质形成细胞上明显缺乏低密度脂蛋白受体，血清胆固醇对表皮甾体生成缺乏影响，这一事实给人的印象是表皮脂质合成可能是自主的，即不可调节的。然而，最近的研究表明，丙酮或洗涤剂对皮肤屏障功能的破坏会刺激表皮固醇生成（J脂质杂志，1985）。为了进一步将甾体生成与屏障功能联系起来，我们测量了必需脂肪酸缺乏（EFAD）无毛小鼠的胆固醇和总非皂化脂质的从头合成。屏障功能有缺陷的动物，表现为经皮失水异常，表皮胆固醇和总非皂化脂合成比对照组增加2倍，而真皮合成不变。在EFAd动物中，系统或局部补充亚油酸后，随着屏障功能的改善，表皮固醇生成恢复正常。此外，EFAD小鼠皮肤的塑料闭塞在第1天和第3天使表皮甾体化正常化。这些结果进一步证明，表皮甾体生成不是完全自主的，可以由水屏障需求调节。
The fact that the skin is a major site of total body sterologensis, coupled both with the apparent absence of low density lipoprotein receptors on keratinocytes and with the lack of influence of serum cholesterol on epidermal sterologensis, has created the impression that epidermal lipid synthesis might be autonomous, i.e. nonregulatable. Recent studies have shown, however, that disruption of cutaneous barrier function with acetone or detergents stimulates epidermal sterologensis (J Lipid Res 26:418–427, 1985). To correlate further sterologenesis with barrier function, we measured de novo synthesis of cholesterol and total nonsaponifiable lipids in essential fatty acid-deficient (EFAD) hairless mice. Animals with defective barrier function, manifested by abnormal transepidermal water loss, demonstrated a 2-fold increase in epidermal cholesterol and total nonsaponifiable lipid synthesis over controls while synthesis in the dermis was unchanged. Epidermal sterologenesis in EFAd animals, repleted with linoleic acid either systematically or topically, returned toward normal as barrier function improved. Moreover, plastic occlusion of EFAD mouse skin normalized epidermal sterologensis at 1 and 3 days. These results provide further evidence that epidermal sterologenesis is not entirely autonomous, and can be regulated by water barrier requirements.