Expression of B7-1 and B7-2 costimulatory molecules by human gastric epithelial cells: potential role in CD4+ T cell activation during Helicobacter pylori infection.
Expression of B7-1 and B7-2 costimulatory molecules by human gastric epithelial cells: potential role in CD4+ T cell activation during Helicobacter pylori infection.
复制标题
人胃上皮细胞表达 B7-1 和 B7-2 共刺激分子：幽门螺杆菌感染期间 CD4 T 细胞激活的潜在作用。
DOI：
10.1172/jci119325
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Reyes,VE
中科院分区：
文献类型：
--
作者：
Ye,G;Barrera,C;Fan,X;Gourley,WK;Crowe,SE;Ernst,PB;Reyes,VE
人胃粘膜上皮细胞显示II类MHC，其表达在幽门螺杆菌感染期间增加。这些观察结果表明胃上皮可能作为抗原递呈细胞（APC）参与局部免疫反应。II类MHC表达的增加与上皮内和上皮附近的胃CD 4 + T细胞数量的升高平行发生。由于APC上B7-1（CD 80）或B7-2（CD 86）的表达是T细胞活化所必需的，因此建立表达这些表面配体的人胃上皮细胞是重要的。用特异性抗体检测B7-1和B7-2在人胃上皮细胞系和新鲜分离的胃活检上皮细胞中的表达。B7-2在蛋白质和转录水平上的表达均高于B7-1，并且在IFN γ处理的上皮细胞上和IFN γ和H.幽门。同样，B7-2在H. pylori感染的组织与未感染的标本进行比较。为了确定这些分子对胃上皮细胞的功能，B7-1和B7-2的抗体显示出降低细胞刺激同种异体反应性CD 4 + T细胞的能力。这些观察结果首次证明B7-1和B7-2在粘膜上皮细胞原位表达。因此，上皮细胞表达B7-1和B7-2可能使它们在调节局部反应中起APC的作用，例如在感染H.幽门。
Human gastric mucosal epithelial cells display class II MHC, the expression of which is increased during Helicobacter pylori infection. These observations suggest that the gastric epithelium may participate as antigen-presenting cells (APC) during local immune responses. The increase in class II MHC expression occurs in parallel with an elevation in gastric CD4+ T cell numbers within and adjacent to the epithelium. Since the expression of either B7-1 (CD80) or B7-2 (CD86) on APC is required for the activation of T cells, it was important to establish human gastric epithelial cells expressed those surface ligands. The expression of B7-1 and B7-2 was detected on human gastric epithelial cell lines and freshly isolated epithelial cells from gastric biopsies with specific antibodies. B7-2 expression was higher than B7-1 at both protein and transcript levels and was increased after crosslinking class II MHC molecules on IFNgamma-treated epithelial cells and in cells pretreated with the combination of IFNgamma and H. pylori. Similarly, B7-2 expression was higher on gastric epithelial cells from H. pylori-infected tissues compared with those from uninfected specimens. To determine the function of these molecules on gastric epithelial cells, antibodies to B7-1 and B7-2 were shown to reduce the ability of the cells to stimulate alloreactive CD4+ T cells. These observations are the first to demonstrate that B7-1 and B7-2 are expressed on mucosal epithelial cells in situ. Thus, the expression of B7-1 and B7-2 by epithelial cells may allow them to act as APC in regulating local responses such as those that occur during infection with H. pylori.