Enhanced biofilm formation and loss of capsule synthesis:: Deletion of a putative glycosyltransferase in Porphyromonas gingivalis
Enhanced biofilm formation and loss of capsule synthesis:: Deletion of a putative glycosyltransferase in Porphyromonas gingivalis
复制标题
DOI：
10.1128/jb.01685-05
复制
发表时间：
2006-08-01
影响因子：
3.2
通讯作者：
Duncan, Margaret J.
中科院分区：
文献类型：
--
作者：
Davey, Mary E.;Duncan, Margaret J.
牙周炎是一种生物膜介导的疾病。牙龈卟啉单胞菌是一种专性厌氧菌一贯与严重的表现，这种疾病。作为一种机会致病菌，其在龈下生物膜（菌斑）内增殖和传播的能力是其毒力的核心。在这里，我们报告的分离牙龈卟啉单胞菌转座子插入突变改变生物膜的发展和重建和表征这种突变在三个不同的野生型菌株。导致生物膜表型改变的突变位于与糖基转移酶基因具有高序列相似性（类似于61%）的基因中。该基因位于染色体的一个区域，该区域包括多达16个预测参与荚膜多糖合成和转运的基因。在所有三种野生型背景中重建的突变的表型是增强的生物膜形成的表型。此外，在菌株W83（一种被包囊的菌株）中，糖基转移酶突变导致包囊丧失。进一步的实验表明，W83突变株更疏水，并表现出增加的自聚集。我们的研究结果表明，我们已经确定了一个基因参与荚膜多糖合成牙龈卟啉单胞菌和生产的胶囊防止附着和启动在聚苯乙烯微量滴定板上的体外生物膜形成。
Periodontitis is a biofilm-mediated disease. Porphyromonas gingivalis is an obligate anaerobe consistently associated with severe manifestations of this disease. As an opportunistic pathogen, the ability to proliferate within and disseminate from subgingival biofilm (plaque) is central to its virulence. Here, we report the isolation of a P. gingivalis transposon insertion mutant altered in biofilm development and the reconstruction and characterization of this mutation in three different wild-type strains. The mutation responsible for the altered biofilm phenotype was in a gene with high sequence similarity (similar to 61%) to a glycosyltransferase gene. The gene is located in a region of the chromosome that includes up to 16 genes predicted to be involved in the synthesis and transport of capsular polysaccharide. The phenotype of the reconstructed mutation in all three wild-type backgrounds is that of enhanced biofilm formation. In addition, in strain W83, a strain that is encapsulated, the glycosyltransferase mutation resulted in a loss of capsule. Further experiments showed that the W83 mutant strain was more hydrophobic and exhibited increased autoaggregation. Our results indicate that we have identified a gene involved in capsular-polysaccharide synthesis in P. gingivalis and that the production of capsule prevented attachment and the initiation of in vitro biofilm formation on polystyrene microtiter plates.