Short Communication: Beta-adrenergic agonists alter oxidative phosphorylation in primary myoblasts.
Short Communication: Beta-adrenergic agonists alter oxidative phosphorylation in primary myoblasts.
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简短的交流：β-肾上腺素能激动剂改变原代成肌细胞的氧化磷酸化。
DOI：
10.1093/jas/skac208
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发表时间：
2022
影响因子：
3.3
通讯作者：
Petersen,JessicaL
中科院分区：
文献类型：
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作者：
Sieck,RenaeL;Treffer,LeahK;Fuller,AnnaM;PonteViana,Martonio;Khalimonchuk,Oleh;Schmidt,TyB;Yates,DustinT;Petersen,JessicaL
β-肾上腺素能受体激动剂（β-AAs）被广泛应用于牛肉和猪肉生产中以提高饲料效率和增加肌肉质量，但对β-AAs实现这一结果的分子机制知之甚少。我们的目的是确定盐酸莱克多巴胺和盐酸齐帕特罗对分离自杂交肉牛（N= 5）、杂交阉牛（N= 2）、约克郡杂交小母猪（N= 3）和商业气候羔羊（N= 5）的肌肉卫星细胞中线粒体呼吸活性的影响。使用Seahorse XFe 24分析仪进行细胞外通量分析，记录耗氧率（OCR）的实时测量值。记录基础OCR测量值后，将盐酸齐帕特罗、盐酸莱克多巴胺或无β-AA进样至试验板中，每种细胞分离株重复三次。然后，将寡霉素、羰腈-对三氟甲氧基苯腙和鱼藤酮依次注射到测定板中，每种诱导不同的细胞状态。这允许在这些状态下测量OCR并计算线粒体功能的以下测量值：基础呼吸、非线粒体呼吸、最大呼吸、质子泄漏、三磷酸腺苷（ATP）相关呼吸和备用呼吸能力。与未补充的对应物相比，用盐酸齐帕特罗或盐酸莱克多巴胺孵育牛细胞增加了最大呼吸（P= 0.046）和备用呼吸能力（P= 0.035）。盐酸齐帕特罗和盐酸莱克多巴胺对牛细胞分离株的最大呼吸量和备用呼吸量无差异（P> 0.05）。在绵羊或猪细胞中，β-AA处理未改变线粒体功能（基础呼吸、非线粒体呼吸、最大呼吸、质子漏、ATP相关呼吸和备用呼吸能力）的测量值。这些结果表明，牛中的β-AAs可能通过改变线粒体呼吸活性来提高肌肉卫星细胞的氧化代谢效率。绵羊和猪细胞对β-AA孵育缺乏反应也证明了不同物种对β-AA的不同生理反应，这有助于解释其作为生长补充剂的有效性的差异。
Beta-adrenergic agonists (β-AAs) are widely used supplements in beef and pork production to improve feed efficiency and increase lean muscle mass, yet little is known about the molecular mechanism by which β-AAs achieve this outcome. Our objective was to identify the influence of ractopamine HCl and zilpaterol HCl on mitochondrial respiratory activity in muscle satellite cells isolated from crossbred beef steers (N= 5), crossbred barrows (N= 2), Yorkshire-cross gilts (N= 3), and commercial weather lambs (N= 5). Real-time measurements of oxygen consumption rates (OCRs) were recorded using extracellular flux analyses with a Seahorse XFe24 analyzer. After basal OCR measurements were recorded, zilpaterol HCl, ractopamine HCl, or no β-AA was injected into the assay plate in three technical replicates for each cell isolate. Then, oligomycin, carbonyl cyanide-p-trifluoromethoxyphenylhydrazone, and rotenone were injected into the assay plate sequentially, each inducing a different cellular state. This allowed for the measurement of OCR at these states and for the calculation of the following measures of mitochondrial function: basal respiration, non-mitochondrial respiration, maximal respiration, proton leak, adenosine triphosphate (ATP)-linked respiration, and spare respiratory capacity. Incubation of bovine cells with either zilpaterol HCl or ractopamine HCl increased maximal respiration (P= 0.046) and spare respiratory capacity (P= 0.035) compared with non-supplemented counterparts. No difference (P> 0.05) was observed between zilpaterol HCl and ractopamine HCl for maximal respiration and spare respiratory capacity in bovine cell isolates. No measures of mitochondrial function (basal respiration, non-mitochondrial respiration, maximal respiration, proton leak, ATP-linked respiration, and spare respiratory capacity) were altered by β-AA treatment in ovine or porcine cells. These findings indicate that β-AAs in cattle may improve the efficiency of oxidative metabolism in muscle satellite cells by modifying mitochondrial respiratory activity. The lack of response by ovine and porcine cells to β-AA incubation also demonstrates differing physiological responses to β-AA across species, which helps to explain the variation in its effectiveness as a growth supplement.