The anti-TMV potency of the tobacco-derived fungus Aspergillus versicolor and its active alkaloids, as anti-TMV activity inhibitors
The anti-TMV potency of the tobacco-derived fungus Aspergillus versicolor and its active alkaloids, as anti-TMV activity inhibitors
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烟草来源的真菌花曲霉及其活性生物碱作为抗 TMV 活性抑制剂的抗 TMV 效力
DOI：
10.1016/j.phytochem.2022.113485
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发表时间：
2022-11-05
期刊：
影响因子：
3.8
通讯作者：
Hu, Qiu-Fen
中科院分区：
文献类型：
--
作者：
Dai, Jia-Meng;Mi, Qi-Li;Hu, Qiu-Fen
烟草（Nicotiana tabacum）作为在中国广泛种植的最重要的经济作物之一引起了人们的兴趣，其干叶被人类社会广泛地用作药用和娱乐。本文报道了5种未描述的生物碱衍生物异曲霉素A-E，以及8种已知生物碱notoamide D、（1 R，4S）-4-苄基-1-异丙基-2，4-二氢-1H-吡嗪并[2，1-B]喹唑啉-3，6-二酮、protuboxepin K、notoamide C、notoamide M、deoxybrevianamide E、cyclo（D-Pro-L-Trp）和versicolamide B从烟草衍生真菌Aspergillus versicolor的培养物中获得。通过对波谱数据的综合分析，主要鉴定了它们的结构。所有分离的化合物的生物活性评价显示，isoaspergilline A和notoamide M表现出抗TMV活性，IC 50值分别为20.0和22.8 μ M。分子对接结果表明，异曲霉素A和notoamide M通过与SER 138、SER 143和ASN 73残基相互作用，很好地定位于抗TMV的活性位点。本研究揭示了内生真菌A.这对寻找未被发现的抗TMV活性抑制剂以及从天然来源中寻找新的抗TMV候选物具有重要意义。
Nicotiana tabacum (tobacco) has attracted interest as one of the most economically important industrial crops widely cultivated in China, whose dried leaves are popularly consumed medicinally and recreationally by human societies. In this study, five undescribed alkaloids derivatives, isoaspergillines A-E, together with eight known alkaloids, notoamide D, (1R,4S)-4-benzyl-1-isopropyl-2,4-dihydro-1H-pyrazino-[2,1-b]quinazoline-3,6-dione, protuboxepin K, notoamide C, notoamide M, deoxybrevianamide E, cyclo (D-Pro-L-Trp), and versicolamide B, were obtained from the culture of the Nicotiana tabacum-derived fungus Aspergillus versicolor. Their structures were mainly elucidated through comprehensive analyses of spectroscopic data. Bioactivity evaluation of all isolated compounds revealed that isoaspergilline A and notoamide M exhibited anti-TMV activities with IC50 values of 20.0 and 22.8 mu M, respectively. Molecular docking suggested that isoaspergilline A and notoamide M were well located into the active site of anti-TMV by interacting with SER138, SER143, and ASN73 residues. This study enlightens the therapeutic potential of the endophytic fungus A. versicolor and it is helpful to find unde-scribed anti-TMV activity inhibitors, as well as searching for new anti-TMV candidates from natural sources.