QUALITATIVE TEST FOR MONITORING COUPLING COMPLETENESS IN SOLID-PHASE PEPTIDE-SYNTHESIS USING CHLORANIL
QUALITATIVE TEST FOR MONITORING COUPLING COMPLETENESS IN SOLID-PHASE PEPTIDE-SYNTHESIS USING CHLORANIL
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DOI：
10.3891/acta.chem.scand.33b-0763
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发表时间：
1979-01-01
期刊：
影响因子：
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通讯作者：
CHRISTENSEN, T
中科院分区：
文献类型：
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作者：
CHRISTENSEN, T
描述了可以在反应混合物存在的情况下进行的定性测试。每隔一段时间，从反应器中取出树脂样品，然后直接进行氯苯胺测试。如果存在游离氨基，树脂珠上会在不到5分钟的时间内形成绿色到蓝色。当N-端氨基酸残基为脯氨酸时，也可以进行检测。通过应用氯苯胺法，证明了偶联率取决于相应的氨基酸和在多肽中的位置。对二环己基碳二亚胺偶联剂和活性酯偶联剂进行了试验。
A qualitative test is described which can be carried out in the presence of the reaction mixture. At intervals, a resin sample is removed from the reactor, and the chloranil test is carried out directly. If free amino groups are present, a green to blue color develops on the resin beads in less than 5 min. The test can also be used when the N-terminal amino acid residue is proline. By applying the chloranil test, it is demonstrated that the coupling rates depend on the respective amino acid and the position in the peptide. The test was applied to dicyclohexylcarbodiimide couplings and an active ester coupling.