Patterned deposition of cells and proteins onto surfaces by using three-dimensional microfluidic systems
Patterned deposition of cells and proteins onto surfaces by using three-dimensional microfluidic systems
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DOI：
10.1073/pnas.040562297
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发表时间：
2000-03-14
影响因子：
11.1
通讯作者：
Whitesides, GM
中科院分区：
文献类型：
--
作者：
Chiu, DT;Jeon, NL;Whitesides, GM
三维微流控系统的制造和用于图案化蛋白质和哺乳动物细胞上的平面基板。邮票中微流体网络的三维拓扑结构使这种技术成为一种通用技术，可以在表面上以复杂的不连续结构对多种类型的蛋白质和细胞进行图案化。当印模与基底表面接触时由印模形成的通道结构限制了通道中细胞的迁移和生长。在通道结构与表面接触的情况下，细胞一旦形成汇合层就停止分裂。去除印章允许细胞扩散和分裂。
Three-dimensional microfluidic systems were fabricated and used to pattern proteins and mammalian cells on a planar substrate. The three-dimensional topology of the microfluidic network in the stamp makes this technique a versatile one with which to pattern multiple types of proteins and cells in complex, discontinuous structures on a surface. The channel structure, formed by the stamp when it is in contact with the surface of the substrate, limits migration and growth of cells in the channels. With the channel structure in contact with the surface, the cells stop dividing once they form a confluent layer. Removal of the stamp permits the cells to spread and divide.