Overexpression of UbcH10 alternates the cell cycle profile and accelerate the tumor proliferation in colon cancer.
Overexpression of UbcH10 alternates the cell cycle profile and accelerate the tumor proliferation in colon cancer.
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DOI：
10.1186/1471-2407-9-87
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发表时间：
2009-03-21
期刊：
影响因子：
3.8
通讯作者：
Doihara H
中科院分区：
文献类型：
--
作者：
Fujita T;Ikeda H;Taira N;Hatoh S;Naito M;Doihara H
UbcH10参与适当的中期到后期的过渡，废除UbcH10的姐妹染色单体的过早分离的结果。为了评估UbcH10在结肠癌进展中的潜在作用，我们分析了UbcH10在结肠癌中的临床病理学相关性。我们首先筛选了UbcH10在各种类型的癌组织以及细胞系中的表达谱。此后，我们使用结肠癌细胞系，操纵UbcH10的表达并评估细胞周期谱和细胞增殖。此外，还对结肠癌患者中UbcH10的临床病理意义进行了免疫组织学评估。使用学生t检验和卡方检验进行统计学分析。使用结肠癌细胞，UbcH10的耗尽导致细胞生长的抑制，而UbcH10的过表达促进细胞生长和致癌细胞生长。有丝分裂人口显着交替UbcH10表达的操纵。免疫组化结果显示UbcH10在结肠癌组织中的表达明显高于正常结肠上皮。此外，临床病理学评估显示，UbcH10与高级别组织学肿瘤相关。结果显示UbcH10在结肠癌进展中的临床病理学意义。因此UbcH10可能作为结肠癌患者的一种新的生物标志物。
UbcH10 participates in proper metaphase to anaphase transition, and abrogation of UbcH10 results in the premature separation of sister chromatids. To assess the potential role of UbcH10 in colon cancer progression, we analyzed the clinicopathological relevance of UbcH10 in colon cancer. We firstly screened the expression profile of UbcH10 in various types of cancer tissues as well as cell lines. Thereafter, using the colon cancer cells line, we manipulated the expression of UbcH10 and evaluated the cell cycle profile and cellular proliferations. Furthermore, the clinicopathological significance of UbcH10 was immunohistologically evaluated in patients with colon cancer. Statistical analysis was performed using the student's t-test and Chi-square test. Using the colon cancer cells, depletion of UbcH10 resulted in suppression of cellular growth whereas overexpression of UbcH10 promoted the cellular growth and oncogenic cellular growth. Mitotic population was markedly alternated by the manipulation of UbcH10 expression. Immunohistochemical analysis indicated that UbcH10 was significantly higher in colon cancer tissue compared with normal colon epithelia. Furthermore, the clinicopathological evaluation revealed that UbcH10 was associated with high-grade histological tumors. The results show the clinicopathological significance of UbcH10 in the progression of colon cancer. Thus UbcH10 may act as a novel biomarker in patients with colon cancer.
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影响因子：
8
作者：
Kienitz, A;Vogel, C;Bastians, H
通讯作者：
Bastians, H
DOI：
10.1073/pnas.94.6.2362
发表时间：
1997-03-18
影响因子：
11.1
作者：
Townsley, FM;Aristarkhov, A;Ruderman, JV
通讯作者：
Ruderman, JV
影响因子：
4
作者：
Walker, Adam;Acquaviva, Claire;Pines, Jonathon
通讯作者：
Pines, Jonathon
影响因子：
21.3
作者：
Hsu, JY;Reimann, JDR;Jackson, PK
通讯作者：
Jackson, PK
影响因子：
56.9
作者：
PAGANO, M;TAM, SW;ROLFE, M
通讯作者：
ROLFE, M