Expression pattern and, surprisingly, gene length shape codon usage in Caenorhabditis, Drosophila, Arabidopsis
Expression pattern and, surprisingly, gene length shape codon usage in Caenorhabditis, Drosophila, Arabidopsis
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DOI：
10.1073/pnas.96.8.4482
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发表时间：
1999-04-13
影响因子：
11.1
通讯作者：
Mouchiroud, D
中科院分区：
文献类型：
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作者：
Duret, L;Mouchiroud, D
我们分别测量了秀丽隐杆线虫、黑腹果蝇和拟南芥中8，133、1，550和2，917个基因的表达模式并分析了密码子使用。在这三个物种中，我们观察到密码子使用和基因表达水平之间存在明显的相关性，并表明这种相关性不是由于突变偏倚。这为在这三种亲缘关系较远的多细胞真核生物中选择沉默位点提供了直接证据。令人惊讶的是，密码子使用和蛋白质长度之间存在很强的负相关性。这种效应不是由于高度表达的蛋白质的较小尺寸。因此，对于相同的表达模式，密码子使用的选择压力似乎在编码长蛋白质的基因中比在编码短蛋白质的基因中低。这一令人困惑的观察结果并没有被任何当前的密码子选择模型所预测，因此提出了翻译效率如何影响多细胞生物体适应性的问题。
We measured the expression pattern and analyzed codon usage in 8,133, 1,550, and 2,917 genes, respectively, from Caenorhabditis elegans, Drosophila melanogaster, and Arabidopsis thaliana. In those three species, we observed a clear correlation between codon usage and gene expression levels and showed that this correlation is not due to a mutational bias. This provides direct evidence for selection on silent sites in those three distantly related multicellular eukaryotes. Surprisingly, there is a strong negative correlation between codon usage and protein length. This effect is not due to a smaller size of highly expressed proteins. Thus, for a same-expression pattern, the selective pressure on codon usage appears to be lower in genes encoding long rather than short proteins. This puzzling observation is not predicted by any of the current models of selection on codon usage and thus raises the question of how translation efficiency affects fitness in multicellular organisms.