Brain distribution of UCP2 mRNA:: In situ hybridization histochemistry studies
Brain distribution of UCP2 mRNA:: In situ hybridization histochemistry studies
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DOI：
10.1038/sj.ijo.0800947
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发表时间：
1999-06-01
影响因子：
4.9
通讯作者：
Ricquier, D
中科院分区：
文献类型：
--
作者：
Richard, D;Huang, Q;Ricquier, D
解偶联蛋白-2(UCP2)在多种组织中大量表达。在小鼠脑中，原位杂交研究发现UCP2在腹中隔区、下丘脑、后脑(延髓)、脑室区和小脑中有丰富的表达。在下丘脑，在视交叉上核、下丘脑室旁核的内侧小细胞和大细胞外侧部分以及弓状核有明显的高强度杂交信号。在脑干中，UCP2在迷走神经的背侧运动核有较强的表达。UCP2基因在脉络膜神经丛和小脑也有明显表达。UCP2 mRNA在大脑特定区域的表达以及它在具有已知化学特性的神经元中的存在表明UCP2 mRNA在神经元中表达。现在就断定UCP2在大脑中的具体功能还为时过早。其转录本的大脑分布模式表明，这种线粒体蛋白可能是参与控制神经内分泌功能和自主反应的神经回路的一部分。假设UCP2 mRNA编码一个功能性解偶联蛋白，可以认为UCP2有助于与其相关的神经元结构的代谢率和体温调节。此外，通过提高大脑的耗氧量，UCP2可以在特定区域控制活性氧物种的产生，从而影响神经退化的过程。
Uncoupling protein-2 (UCP2) is expressed in large amounts in several tissues. In the mouse brain, in situ hybridization studies have revealed an abundant expression of UCP2 mRNA in the ventral septal region, the hypothalamus, the hindbrain (medulla), the ventricular regions and the cerebellum. In the hypothalamus, a very highly intense hybridization signal is apparent in the suprachiasmatic nucleus, in the medial parvicellular and magnocellular lateral parts of the paraventricular hypothalamic nucleus, and in the arcuate nucleus. In the brainstem, UCP2 is found to be strongly expressed in the dorsal motor nucleus of the vagus nerve. The expression of UCP2 mRNA is also clearly noticeable in the choroid plexuses and in the cerebellum. The expression of UCP2 mRNA in specific regions of the brain as well as its presence in neurons with a known chemical identity suggest that UCP2 mRNA is expressed in neurons. It is as yet premature to conclude about a specific function of UCP2 in the brain. The brain distribution pattern of its transcript suggests that this mitochondrial protein could be part of neuronal circuitries involved in the control of neuroendocrine functions and autonomic responses. Assuming that the UCP2 mRNA encodes a functional uncoupling protein, it can be argued that UCP2 contributes to the metabolic rate and thermoregulation of the neuronal structures to which it is associated. In addition, by elevating oxygen consumption in the brain, UCP2 could in specific regions control the production of reactive oxygen species and thereby influence the process of neural degeneration.