Measurement of precursors for alpha-amidated hormones by radioimmunoassay of glycine-extended peptides after trypsin-carboxypeptidase B cleavage.
Measurement of precursors for alpha-amidated hormones by radioimmunoassay of glycine-extended peptides after trypsin-carboxypeptidase B cleavage.
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通过胰蛋白酶-羧肽酶 B 裂解后甘氨酸延伸肽的放射免疫测定法测量 α-酰胺化激素的前体。
DOI：
10.1016/0003-2697(86)90129-6
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发表时间：
1986
影响因子：
2.9
通讯作者：
J. Rehfeld
中科院分区：
文献类型：
--
作者：
L. Hilsted;J. Rehfeld
使用片段5-17的人胃泌素-17扩展与甘氨酸在C-末端作为半抗原，三个6只兔子产生高滴度，高亲和力的抗血清特异性甘氨酸扩展的胃泌素。结合胰蛋白酶和羧肽酶B裂解，基于这些抗血清的放射免疫测定法测量了一些胃窦外组织和某些恶性肿瘤中的前胃泌素。结果表明，胰蛋白酶和羧肽酶B连续切割，然后进行甘氨酸延伸肽的放射免疫测定是一种快速和准确的方法，用于测量α-酰胺化肽激素的生物合成前体。此外，该方法在寻找肿瘤标志物方面似乎很有前途。
Using fragment 5–17 of human gastrin-17 extended with glycine at the C-terminus as hapten, three of six rabbits produced high-titer, high-avidity antisera specific for glycine-extended gastrins. In combination with trypsin and carboxypeptidase B cleavage, radioimmunoassays based on these antisera measured progastrins in some extra-antral tissues and certain malignant tumors. The results show that sequential cleavage with trypsin and carboxypeptidase B followed by radioimmunoassay of glycine-extended peptides is a rapid and accurate procedure for measurement of biosynthetic precursors of α-amidated peptide hormones. Moreover, the procedure seems promising in the search for tumor markers.