p16 mutation frequency and clinical correlation in head and neck cancer.
p16 mutation frequency and clinical correlation in head and neck cancer.
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DOI：
10.1080/00016489950181837
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发表时间：
1999-03
影响因子：
1.4
通讯作者：
Daniel G. Danahey;Evan J. Tobin;David E. Schuller;Carol M. Bier-Laning;C. Weghorst;Jas C. Lang
中科院分区：
文献类型：
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作者：
Daniel G. Danahey;Evan J. Tobin;David E. Schuller;Carol M. Bier-Laning;C. Weghorst;Jas C. Lang
肿瘤抑制基因p16被改变后，已被证明在包括头颈癌在内的许多不同肿瘤类型的肿瘤发生中发挥作用。这项研究的目的是分析头颈部鳞状细胞癌(SCC)中p16的变化，并将其与临床结果相关联。从26例头颈部鳞状细胞癌肿瘤和24例配对对照中提取RNA。逆转录-聚合酶链式反应扩增p16基因，测序并分析其突变情况。在26例患者中，58%的肿瘤有p16基因改变，主要表现为：8例缺失，1例插入/缺失，4例点突变，2例无p16表达。在24个匹配的正常组织样本中，没有发现p16基因的改变。那些p16基因改变的患者的存活率似乎与那些没有p16基因改变的患者相当，尽管p16基因改变的患者似乎有更多的复发。
The tumor suppressor gene p16, when altered, has been shown to play a role in oncogenesis in many different tumor types including head and neck cancer. The goal of this study was to analyse alterations to p16 in squamous cell carcinoma (SCC) of the head and neck and to correlate these with clinical outcome. RNA was isolated from 26 SCC head and neck tumors and from 24 matched controls. A reverse transcription polymerase chain reaction was utilized to generate p16 cDNA, which was sequenced and analysed for alterations. In the 26 patient group 58% of the tumors had a p16 alteration, which were characterized by: 8 deletions, 1 insertion/deletion, 4 point mutations and 2 with no p16 expression. In 24 matched normal tissue samples there were no p16 alterations. Those patients with p16 alterations appear to have survival rates comparable to those without p16 alterations, although patients with p16 alterations appear to have more recurrences.