Genomic insight into a novel actinobacterium, Actinomadura rubrisoli sp. nov., reveals high potential for bioactive metabolites.
Genomic insight into a novel actinobacterium, Actinomadura rubrisoli sp. nov., reveals high potential for bioactive metabolites.
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对一种新型放线菌（Actinomadura rubrisoli sp）的基因组见解。
DOI：
10.1007/s10482-020-01511-5
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Ay H
中科院分区：
文献类型：
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作者：
Ay H
从未开发的栖息地中分离新型放线菌作为新型药物先导的潜在来源至关重要。在对干旱土壤样品进行新型放线菌筛选的过程中，从土耳其Malatya分离到菌株h3c3t，并利用基因组多相方法确定了菌株的分类位置。16S rRNA基因的两两序列比较表明，该菌株与纤维放线瘤jcm 9371亲缘关系密切，序列同源性为99.0%。基于数字DNA-DNA杂交和平均核苷酸鉴定的比较基因组分析表明，菌株h3c3代表了放线瘤属的一个新种。该菌株具有典型的放线菌属特征，即诊断氨基酸为中二氨基亚苯甲酸；半乳糖，葡萄糖，麦芽糖和核糖作为全细胞糖。检测到的甲基萘醌类主要为MK-9（H6）、MK-9(H8)，极性脂类主要为二磷脂酰甘油、磷脂酰肌醇、糖磷脂及未知磷脂和脂类。其基因组大小约为102m， G+C含量为71.6%。进一步的基因组分析表明，菌株h3c3t极有可能成为编码多种化学结构的新型生物合成基因簇。在表型和系统发育分析的基础上，菌株h3c3代表了放线瘤属（actinomadura rubrisolisp）的一个新种。11月被提出，它对生物制药工业有价值的新型生物合成代谢物具有很高的希望。
Isolation of novel actinobacteria from unexplored habitats as potential sources of novel drug leads has utmost importance. During the course of screening arid soil samples for novel actinobacteria, strain H3C3Twas isolated from Malatya, Turkey and its taxonomic position was revealed by a genome-based polyphasic approach. Pairwise sequence comparison of the 16S rRNA gene showed that the strain is closely related toActinomadura fibrosaJCM 9371Twith sequence identity level of 99.0%. Comparative genome analyses based on digital DNA-DNA hybridization and average nucleotide identity indicated that strain H3C3Trepresents a novel species within the genusActinomadura. The strain has typical characteristics of the genusActinomadura, i.e. meso-diaminopimelic acid as diagnostic amino acid; galactose, glucose, madurose and ribose as whole-cell sugars. Major menaquinones detected were MK-9(H6), MK-9(H8) and polar lipids were diphosphatidylglycerol, phosphatidylinositol, glycophospholipid and unknown phospholipid and lipids. Its genome size is approximately 10.2 Mb with G+C content of 71.6%. Further genomic analyses of strain H3C3Tindicated its high potential for novel biosynthetic gene clusters coding for various chemical structures. On the basis of phenotypic and phylogenetic analyses, strain H3C3Trepresents a novel species of the genusActinomadura, for whichActinomadura rubrisolisp. nov. is proposed, and it holds high promise for novel biosynthetic metabolites of value to biopharmaceutical industry.