Barbiturate-induced expression of neuronal nitric oxide synthase in the rat cerebellum.
Barbiturate-induced expression of neuronal nitric oxide synthase in the rat cerebellum.
复制标题
巴比妥诱导大鼠小脑神经元一氧化氮合酶的表达。
DOI：
10.1016/s0006-8993(97)00057-7
复制
发表时间：
1997
期刊：
影响因子：
2.9
通讯作者：
Strobel,HW
中科院分区：
文献类型：
--
作者：
Thompson,CM;Bernhard,AE;Strobel,HW
神经元型一氧化氮合酶（nNOS）与细胞色素P450混合功能氧化酶系统在结构和功能上有相似之处。与P450不同，它不需要第二种酶还原酶来转移电子。该特性与巨大芽孢杆菌P450 BM-3相似。P450 BM-3和某些哺乳动物P450亚家族，如P4502 B，已知被苯巴比妥（PB）诱导，这些P450共享一个称为芭比盒的共有序列。由于nNOS与P450 BM-3和其他哺乳动物P450的相似性，我们已经研究了nNOS是否也对PB治疗有反应。我们使用半定量PCR、蛋白质印迹分析、功能测定和免疫组织化学来回答这个问题。这些数据显示，PB治疗后nNOS mRNA表达增加三倍，nNOS蛋白和活性诱导更温和，并且nNOS在小脑内的定位没有明显变化。
Neuronal nitric oxide synthase (nNOS) is known to share some structural and functional similarities with the cytochrome P450 mixed function oxidase system. Unlike P450, it does not require a second enzyme, reductase, to transfer electrons. This characteristic is similar to P450BM-3of Bacillus megaterium. P450BM-3and certain mammalian subfamilies of P450, such as P4502B, are known to be induced by phenobarbital (PB), and these P450s share a consensus sequence called the Barbie box. Because of the similarities nNOS shares with P450BM-3and other mammalian P450s, we have examined whether nNOS also responds to PB treatment. We have used semi-quantitative PCR, Western blot analysis, a functional assay, and immunohistochemistry in order to answer this question. These data show a threefold increase in nNOS mRNA expression, more modest nNOS protein and activity induction, and no discernible changes in localization of nNOS within the cerebellum following PB treatment.