Determination of Functional Activity of Human iPSC-Derived Hepatocytes by Measurement of CYP Metabolism.
Determination of Functional Activity of Human iPSC-Derived Hepatocytes by Measurement of CYP Metabolism.
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通过测量 CYP 代谢测定人 iPSC 来源的肝细胞的功能活性。
DOI：
10.1007/7651_2014_145
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Jang,Yoon-Young
中科院分区：
文献类型：
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作者：
Chaudhari,Pooja;Prasad,Neha;Tian,Lipeng;Jang,Yoon-Young
诱导多能干细胞 (iPSC) 技术的出现使得一系列特定人类疾病表型的建模成为可能，有助于对疾病进展和发病机制日益重要且不可或缺的理解。多能干细胞来源的肝细胞为精准医学的药物筛选和个性化药物测试提供了新途径。 CYP450微粒体酶在药物代谢中发挥着关键作用。因此，iPSC 来源的肝细胞的 CYP 活性测量是进行药物测试之前确保代谢功能的重要先决条件。在此，我们描述了测量人类 iPSC 衍生肝细胞中不同 CYP450 酶活性的方案。
The advent of induced pluripotent stem cell (iPSC) technology has enabled the modeling of an array of specific human disease phenotypes, aiding in the increasingly important and indispensable understanding of disease progression and pathogenesis. Pluripotent stem cell-derived hepatocytes present a new avenue for drug screening and personalized drug testing toward precision medicine. CYP450 microsomal enzymes play a critical role in drug metabolism. Hence, CYP activity measurement of iPSC-derived hepatocytes is a vital prerequisite, to ensure metabolic functionality before proceeding to drug testing. Herein, we describe the protocol for measurement of different CYP450 enzyme activities in human iPSC-derived hepatocytes.