Developmental changes of TGF-β1 and Smads signaling pathway in intestinal adaption of weaned pigs.
Developmental changes of TGF-β1 and Smads signaling pathway in intestinal adaption of weaned pigs.
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断奶仔猪肠道适应中TGF-β1和Smads信号通路的发育变化
DOI：
10.1371/journal.pone.0104589
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Hu CH
中科院分区：
文献类型：
--
作者：
Xiao K;Song ZH;Jiao LF;Ke YL;Hu CH
断奶应激引起肠道结构和功能的明显变化。转化生长因子-β1 （TGF-β1）和典型Smads信号通路被怀疑在小肠断奶后适应中发挥重要的调节作用。本研究对断奶后2周仔猪肠道形态、通透性、小肠紧密连接蛋白和TGF-β1的发育表达进行了研究。通过检测TGF-β受体I/II （TβRI、TβRII）、smad2/3、smad4和smad7的表达，探讨典型smads信号通路是否参与早期断奶适应过程。结果表明，断奶后第3天和第7天肠道绒毛变短，隐窝变深，断奶后第14天肠道形态恢复到断奶前水平。早期断奶提高了断奶后第3、7天肠黏膜二胺氧化酶（DAO）水平（P<0.05），降低了DAO活性（P<0.05）。与断奶前（第0天）相比，occludin和claudin-1紧密连接蛋白水平在断奶后第3、7和14天降低（P<0.05）， ZO-1蛋白水平在断奶后第3和7天降低（P<0.05）。断奶后第3、7天肠黏膜TGF-β1水平升高（P<0.05），第14天下降。断奶后肠黏膜TβRII蛋白表达量在d3和d7时均有升高（P<0.05），但TβRI、TβRII、smad2/3、smad4和smad7 mRNA表达量无显著升高。结果表明，TGF-β1与断奶应激后肠道形态和屏障功能的恢复有关。肠道内源性TGF-β1的增加并未激活典型的Smads信号通路。
Weaning stress caused marked changes in intestinal structure and function. Transforming growth factor-β1 (TGF-β1) and canonical Smads signaling pathway are suspected to play an important regulatory role in post-weaning adaptation of the small intestine. In the present study, the intestinal morphology and permeability, developmental expressions of tight junction proteins and TGF-β1 in the intestine of piglets during the 2 weeks after weaning were assessed. The expressions of TGF-β receptor I/II (TβRI, TβRII), smad2/3, smad4 and smad7 were determined to investigate whether canonical smads signaling pathways were involved in early weaning adaption process. The results showed that a shorter villus and deeper crypt were observed on d 3 and d 7 postweaning and intestinal morphology recovered to preweaning values on d 14 postweaning. Early weaning increased (P<0.05) plasma level of diamine oxidase (DAO) and decreased DAO activities (P<0.05) in intestinal mucosa on d 3 and d 7 post-weaning. Compared with the pre-weaning stage (d 0), tight junction proteins level of occludin and claudin-1 were reduced (P<0.05) on d 3, 7 and 14 post-weaning, and ZO-1 protein was reduced (P<0.05) on d 3 and d 7 post-weaning. An increase (P<0.05) of TGF-β1 in intestinal mucosa was observed on d 3 and d 7 and then level down on d 14 post-weaning. Although there was an increase (P<0.05) of TβR II protein expression in the intestinal mucosa on d3 and d 7, no significant increase of mRNA of TβRI, TβRII, smad2/3, smad4 and smad7 was observed during postweaning. The results indicated that TGF-β1 was associated with the restoration of intestinal morphology and barrier function following weaning stress. The increased intestinal endogenous TGF-β1 didn't activate the canonical Smads signaling pathway.
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影响因子：
7.7
作者：
Chen, Hai-hua;Zhou, Xian-long;Yang, Jiong
通讯作者：
Yang, Jiong
影响因子：
4.2
作者：
Hering, Nina A.;Andres, Susanne;Schulzke, Joerg D.
通讯作者：
Schulzke, Joerg D.
DOI：
10.1152/ajpgi.00304.2006
发表时间：
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影响因子：
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作者：
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通讯作者：
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影响因子：
4.1
作者：
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通讯作者：
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影响因子：
29.4
作者：
DIGNASS, AU;PODOLSKY, DK
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PODOLSKY, DK